Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917707" target="_blank" >RIV/62156489:43210/20:43917707 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/20:PU136548

Výsledek na webu

<a href="https://doi.org/10.1016/j.foodchem.2020.126673" target="_blank" >https://doi.org/10.1016/j.foodchem.2020.126673</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodchem.2020.126673" target="_blank" >10.1016/j.foodchem.2020.126673</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

Popis výsledku v původním jazyce

In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting. Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 x 103 CFU.mlMINUS SIGN 1, which corresponds to the limit set in European Union legislation for microbial control of food.

Název v anglickém jazyce

Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus

Popis výsledku anglicky

In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting. Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 x 103 CFU.mlMINUS SIGN 1, which corresponds to the limit set in European Union legislation for microbial control of food.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Chemistry

ISSN

0308-8146

e-ISSN

—

Svazek periodika

321

Číslo periodika v rámci svazku

15 August

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

126673

Kód UT WoS článku

000526394400008

EID výsledku v databázi Scopus

2-s2.0-85082852951