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Diclofenac-Induced Cytotoxicity in Cultured Carp Leukocytes

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919373" target="_blank" >RIV/62156489:43210/20:43919373 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/00216224:14310/20:00118436 RIV/62157124:16270/20:43879041

  • Výsledek na webu

    <a href="https://doi.org/10.33549/physiolres.934609" target="_blank" >https://doi.org/10.33549/physiolres.934609</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.33549/physiolres.934609" target="_blank" >10.33549/physiolres.934609</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Diclofenac-Induced Cytotoxicity in Cultured Carp Leukocytes

  • Popis výsledku v původním jazyce

    Diclofenac is a drug commonly used in human and veterinary medicine for the treatment of diseases associated with inflammation and pain. Medicinal products enter waste and surface waters on an everyday basis and contaminate the aquatic environment. Fish are therefore permanently exposed to these chemicals dissolved in their aquatic environment. To simulate variable environmental conditions, the aim of our study was to examine adverse effects of diclofenac under different temperatures of cell incubation (18, 21, 24, 27 and 30 degrees C). Cytotoxic and -static effects of diclofenac in concentrations of 0.001 mu g/ml, 0.01 mu g/ml, 0.1 mu g/ml, 1 mu g/ml, 10 mu g/ml and 100 mu g/ml for the carp (Cyprinus carpio) cultured leukocytes were quantified using detection of lactate dehydrogenase released from damaged cells. Overall DCF cytotoxicity was relatively low and its impact was pronounced at higher temperature and DCF concentration. Cells growth inhibition is changing more rapidly but it is high mainly at the highest concentration from low temperature. DNA fragmentation was not detected in tested leukocyte cell line. CYP450 increased diclofenac cytotoxicity only at the highest concentration but at incubation temperatures 18 and 27 degrees C. Leukocyte viability is essential for immune functions and any change can lead to reduction of resistance against pathogens, mainly in cold year seasons, when the immune system is naturally suppressed.

  • Název v anglickém jazyce

    Diclofenac-Induced Cytotoxicity in Cultured Carp Leukocytes

  • Popis výsledku anglicky

    Diclofenac is a drug commonly used in human and veterinary medicine for the treatment of diseases associated with inflammation and pain. Medicinal products enter waste and surface waters on an everyday basis and contaminate the aquatic environment. Fish are therefore permanently exposed to these chemicals dissolved in their aquatic environment. To simulate variable environmental conditions, the aim of our study was to examine adverse effects of diclofenac under different temperatures of cell incubation (18, 21, 24, 27 and 30 degrees C). Cytotoxic and -static effects of diclofenac in concentrations of 0.001 mu g/ml, 0.01 mu g/ml, 0.1 mu g/ml, 1 mu g/ml, 10 mu g/ml and 100 mu g/ml for the carp (Cyprinus carpio) cultured leukocytes were quantified using detection of lactate dehydrogenase released from damaged cells. Overall DCF cytotoxicity was relatively low and its impact was pronounced at higher temperature and DCF concentration. Cells growth inhibition is changing more rapidly but it is high mainly at the highest concentration from low temperature. DNA fragmentation was not detected in tested leukocyte cell line. CYP450 increased diclofenac cytotoxicity only at the highest concentration but at incubation temperatures 18 and 27 degrees C. Leukocyte viability is essential for immune functions and any change can lead to reduction of resistance against pathogens, mainly in cold year seasons, when the immune system is naturally suppressed.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    40103 - Fishery

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Physiological Research

  • ISSN

    0862-8408

  • e-ISSN

  • Svazek periodika

    69

  • Číslo periodika v rámci svazku

    Supplement 4

  • Stát vydavatele periodika

    CZ - Česká republika

  • Počet stran výsledku

    12

  • Strana od-do

    "S607"-"S618"

  • Kód UT WoS článku

    000621838200007

  • EID výsledku v databázi Scopus

    2-s2.0-85102229899