The use of molecular-genetic methods and bioinformatics tools to identify the representatives of the order Coleoptera occurring on decaying cadavers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919588" target="_blank" >RIV/62156489:43210/20:43919588 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The use of molecular-genetic methods and bioinformatics tools to identify the representatives of the order Coleoptera occurring on decaying cadavers

Popis výsledku v původním jazyce

The process of gradual settlement of the dead body by necrophagous insects plays an important role in forensic practice, especially when determining the time of death. This work deals with the decomposition of dead body of vertebrates by forensically important representatives from the order of Coleoptera in the Czech Republic. Experimental objects for our purposes were domestic pig and chicken. The research was realized from November 2017 to March 2019. During the study samples of larvae and adults were collected from the dead bodies and subsequently identified molecular-genetic methods. For research purposes was necessary to isolate the genomic DNA, amplify the fragment of cytochrome c oxidase I (COI) gene by the PCR method and sequencing this fragment DNA. The BLAST and BOLD databases showed the same outputs and based on the results the individuals were classified into individual families of the Coleoptera order.

Název v anglickém jazyce

The use of molecular-genetic methods and bioinformatics tools to identify the representatives of the order Coleoptera occurring on decaying cadavers

Popis výsledku anglicky

The process of gradual settlement of the dead body by necrophagous insects plays an important role in forensic practice, especially when determining the time of death. This work deals with the decomposition of dead body of vertebrates by forensically important representatives from the order of Coleoptera in the Czech Republic. Experimental objects for our purposes were domestic pig and chicken. The research was realized from November 2017 to March 2019. During the study samples of larvae and adults were collected from the dead bodies and subsequently identified molecular-genetic methods. For research purposes was necessary to isolate the genomic DNA, amplify the fragment of cytochrome c oxidase I (COI) gene by the PCR method and sequencing this fragment DNA. The BLAST and BOLD databases showed the same outputs and based on the results the individuals were classified into individual families of the Coleoptera order.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10616 - Entomology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2020: Proceedings of International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

435-438

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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