Barcoding analysis for identification of insect species on decaying remains
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43924753" target="_blank" >RIV/62156489:43210/23:43924753 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43410/23:43924753
Výsledek na webu
<a href="https://doi.org/10.15414/afz.2023.26.04.347-353" target="_blank" >https://doi.org/10.15414/afz.2023.26.04.347-353</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15414/afz.2023.26.04.347-353" target="_blank" >10.15414/afz.2023.26.04.347-353</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Barcoding analysis for identification of insect species on decaying remains
Popis výsledku v původním jazyce
Identifying necrophagous individuals on a dead body is most often associated with forensic practice and the field of forensic entomology. Insects are found on a dead body depending on climatic conditions, with the seasons naturally accelerating the decomposition of tissues and successive waves gradually reducing the amount of soft tissue remaining. The aim of this work was to compare the composition of the necrophagous invertebrate species occurring on different carcasses (Gallus gallus vs Sus scrofa f. domestica). A second objective was to evaluate which of the identification tools comparing COI gene sequences is currently more appropriate for barcoding and whether there are significant differences in the results of the BOLD and BLAST identification tools between the samples examined. Molecular determination was done using Sanger sequencing of the COI fragment of mitochondrial DNA. In total, 96 individuals and 13 species were identified in the Gallus gallus carcass and 50 individuals and 17 species on the Sus scrofa (f. domestica) carcass. Calliphora vomitoria was determined as the most abundant species in both corpses'remains. The reliability of the BOLD and BLAST identification methods is comparably high.
Název v anglickém jazyce
Barcoding analysis for identification of insect species on decaying remains
Popis výsledku anglicky
Identifying necrophagous individuals on a dead body is most often associated with forensic practice and the field of forensic entomology. Insects are found on a dead body depending on climatic conditions, with the seasons naturally accelerating the decomposition of tissues and successive waves gradually reducing the amount of soft tissue remaining. The aim of this work was to compare the composition of the necrophagous invertebrate species occurring on different carcasses (Gallus gallus vs Sus scrofa f. domestica). A second objective was to evaluate which of the identification tools comparing COI gene sequences is currently more appropriate for barcoding and whether there are significant differences in the results of the BOLD and BLAST identification tools between the samples examined. Molecular determination was done using Sanger sequencing of the COI fragment of mitochondrial DNA. In total, 96 individuals and 13 species were identified in the Gallus gallus carcass and 50 individuals and 17 species on the Sus scrofa (f. domestica) carcass. Calliphora vomitoria was determined as the most abundant species in both corpses'remains. The reliability of the BOLD and BLAST identification methods is comparably high.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
10616 - Entomology
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta Fytotechnica et Zootechnica
ISSN
1335-258X
e-ISSN
1336-9245
Svazek periodika
26
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
SK - Slovenská republika
Počet stran výsledku
7
Strana od-do
347-353
Kód UT WoS článku
—
EID výsledku v databázi Scopus
2-s2.0-85182876668