Identification of volatile compounds associated with sepsis progression by mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919610" target="_blank" >RIV/62156489:43210/20:43919610 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of volatile compounds associated with sepsis progression by mass spectrometry

Popis výsledku v původním jazyce

Herein, SPMEMINUS SIGN GCxGCMINUS SIGN TOFMS method was used to optimize the parameters of the method and correct conditions for sample processing which will allow to develop a method for the identification of VOCs (mainly alcohol, ketones and hydrocarbons) sepsis biomarkers. The correct conditions for processing the samples (the incubation - heating/collection of samples, the selection of SPME fiber for sample collection and the addition of NaCl) were established. Moreover, in this work, the GCMINUS SIGN MS (Pegasus 4DMINUS SIGN C) method was used. Parameters of the chromatography and mass spectrometry methods GCxGCMINUS SIGN TOFMS were tested and optimized (temperature of the secondary oven, modulator temperature, modulator period, cool time etc.). Altogether, about 5 methods with different settings were compared. Finally, the best combination of the optimized sample conditions and optimized method was developed. The heating and collection of the sample 30min/30min by the DVB/CAR/PDMS fiber in the addition of NaCl (200 mg/ml) were chosen. Moreover, one of the 5 optimized GCxGCMINUS SIGN TOFMS method was selected. The optimized method will be used for analyses of monocyte from serum samples.

Název v anglickém jazyce

Identification of volatile compounds associated with sepsis progression by mass spectrometry

Popis výsledku anglicky

Herein, SPMEMINUS SIGN GCxGCMINUS SIGN TOFMS method was used to optimize the parameters of the method and correct conditions for sample processing which will allow to develop a method for the identification of VOCs (mainly alcohol, ketones and hydrocarbons) sepsis biomarkers. The correct conditions for processing the samples (the incubation - heating/collection of samples, the selection of SPME fiber for sample collection and the addition of NaCl) were established. Moreover, in this work, the GCMINUS SIGN MS (Pegasus 4DMINUS SIGN C) method was used. Parameters of the chromatography and mass spectrometry methods GCxGCMINUS SIGN TOFMS were tested and optimized (temperature of the secondary oven, modulator temperature, modulator period, cool time etc.). Altogether, about 5 methods with different settings were compared. Finally, the best combination of the optimized sample conditions and optimized method was developed. The heating and collection of the sample 30min/30min by the DVB/CAR/PDMS fiber in the addition of NaCl (200 mg/ml) were chosen. Moreover, one of the 5 optimized GCxGCMINUS SIGN TOFMS method was selected. The optimized method will be used for analyses of monocyte from serum samples.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2020: Proceedings of International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

548-552

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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