In vitro antioxidant and antibacterial activities with polyphenolic profling of wild cherry, the European larch and sweet chestnut tree bark

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919928" target="_blank" >RIV/62156489:43210/21:43919928 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s00217-021-03796-w" target="_blank" >https://doi.org/10.1007/s00217-021-03796-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00217-021-03796-w" target="_blank" >10.1007/s00217-021-03796-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro antioxidant and antibacterial activities with polyphenolic profling of wild cherry, the European larch and sweet chestnut tree bark

Popis výsledku v původním jazyce

This study is a comparative investigation of antioxidant and antibacterial properties of tree bark extracts of three common European species, Prunus avium L., Larix decidua Mill. and Castanea sativa Mill. The bioactive compounds present in the bark were recovered in 80% aqueous ethanol using ultrasound as the green extraction method. The antioxidant potential of the extracts was assessed with multiple biochemical assays: total phenol content (TPC) expressed in gallic acid equivalent (GAE), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2&apos;-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) expressed in trolox equivalent (TE), and ferric reducing ability of plasma (FRAP) expressed in ascorbic acid equivalent (AAE). Sweet chestnut bark extract showed the highest antioxidant activity with TPC of 174.25+-16.95 mg GAE/g dry weight, DPPH (IC50) of 2.69+-0.03 μg/mL, ABTS of 739.65+-24.41 mg TE/g dry weight and FRAP of 207.49+-3.62 mg AAE/g dry weight. The antibacterial activity of the extracts was evaluated by disk difusion test, minimal inhibitory concentration (MIC) assay and bacterial growth curves. Sweet chestnut bark extract gave IC50 values of 0.25 mg/mL and 1.00 mg/mL against E. coli and S. aureus, respectively. The polyphenolic profling of the bark extracts was performed to identify the major compounds responsible for the bioactivities using high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS). The bark extracts were rich in natural antioxidants, thus holding tremendous potential for use as natural additives in food industry.

Název v anglickém jazyce

In vitro antioxidant and antibacterial activities with polyphenolic profling of wild cherry, the European larch and sweet chestnut tree bark

Popis výsledku anglicky

This study is a comparative investigation of antioxidant and antibacterial properties of tree bark extracts of three common European species, Prunus avium L., Larix decidua Mill. and Castanea sativa Mill. The bioactive compounds present in the bark were recovered in 80% aqueous ethanol using ultrasound as the green extraction method. The antioxidant potential of the extracts was assessed with multiple biochemical assays: total phenol content (TPC) expressed in gallic acid equivalent (GAE), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2&apos;-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) expressed in trolox equivalent (TE), and ferric reducing ability of plasma (FRAP) expressed in ascorbic acid equivalent (AAE). Sweet chestnut bark extract showed the highest antioxidant activity with TPC of 174.25+-16.95 mg GAE/g dry weight, DPPH (IC50) of 2.69+-0.03 μg/mL, ABTS of 739.65+-24.41 mg TE/g dry weight and FRAP of 207.49+-3.62 mg AAE/g dry weight. The antibacterial activity of the extracts was evaluated by disk difusion test, minimal inhibitory concentration (MIC) assay and bacterial growth curves. Sweet chestnut bark extract gave IC50 values of 0.25 mg/mL and 1.00 mg/mL against E. coli and S. aureus, respectively. The polyphenolic profling of the bark extracts was performed to identify the major compounds responsible for the bioactivities using high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS). The bark extracts were rich in natural antioxidants, thus holding tremendous potential for use as natural additives in food industry.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/EF16_025%2F0007314" target="_blank" >EF16_025/0007314: Multioborový výzkum pro zvýšení aplikačního potenciálu nanomateriálů v zemědělské praxi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Food Research and Technology

ISSN

1438-2377

e-ISSN

—

Svazek periodika

247

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

16

Strana od-do

2355-2370

Kód UT WoS článku

000660788800001

EID výsledku v databázi Scopus

2-s2.0-85107792712