Associations of SOST and TNFSF11 genes polymorphisms with bone parameters in broilers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921822" target="_blank" >RIV/62156489:43210/21:43921822 - isvavai.cz</a>

Výsledek na webu

<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Associations of SOST and TNFSF11 genes polymorphisms with bone parameters in broilers

Popis výsledku v původním jazyce

In modern broiler lines, increasing demands are placed on skeletal integrity, where these lines are often characterized by poor calcification and high bone porosity. Bone abnormalities, as disruption of the normal skeletal growth process and homeostasis, are usually complex and lead to increased affinity for bone damage and many bone diseases. Bone quality traits can be improved by genetic tools, given the additive genetic background of these traits. In this study, attention was focused mainly on the search for polymorphisms in selected candidate genes that play an important role in bone metabolism. We studied the associations of single nucleotide polymorphisms in several regions of two genes (SOST and TNFSF11) with selected bone parameters (bone breaking strength, length, width, bone mass) in fast (Ross 308) and slow (Hubbard M22BxJA87A) growing broilers, whereas the individual hybrid lines were also compared on the basis of the monitored bone parameters. A total of 48 animals were tested. PCR and sequencing were used to determine the polymorphisms. In selected regions of studied genes, thirteen polymorphisms have been discovered. Five of these polymorphisms were in the introns and three were synonymous. Only one polymorphism was found in selected gene regions in the experimental group of animals, which showed associations with bone breaking strength, but this polymorphism was found in the intron. Other polymorphisms, especially those found in the exon, did not show associations with the observed bone parameters.

Název v anglickém jazyce

Associations of SOST and TNFSF11 genes polymorphisms with bone parameters in broilers

Popis výsledku anglicky

In modern broiler lines, increasing demands are placed on skeletal integrity, where these lines are often characterized by poor calcification and high bone porosity. Bone abnormalities, as disruption of the normal skeletal growth process and homeostasis, are usually complex and lead to increased affinity for bone damage and many bone diseases. Bone quality traits can be improved by genetic tools, given the additive genetic background of these traits. In this study, attention was focused mainly on the search for polymorphisms in selected candidate genes that play an important role in bone metabolism. We studied the associations of single nucleotide polymorphisms in several regions of two genes (SOST and TNFSF11) with selected bone parameters (bone breaking strength, length, width, bone mass) in fast (Ross 308) and slow (Hubbard M22BxJA87A) growing broilers, whereas the individual hybrid lines were also compared on the basis of the monitored bone parameters. A total of 48 animals were tested. PCR and sequencing were used to determine the polymorphisms. In selected regions of studied genes, thirteen polymorphisms have been discovered. Five of these polymorphisms were in the introns and three were synonymous. Only one polymorphism was found in selected gene regions in the experimental group of animals, which showed associations with bone breaking strength, but this polymorphism was found in the intron. Other polymorphisms, especially those found in the exon, did not show associations with the observed bone parameters.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2021: Proceedings of International PhD Students Conference

ISBN

978-80-7509-821-4

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

399-404

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

10. 11. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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