A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43923409" target="_blank" >RIV/62156489:43210/23:43923409 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/23:00133292

Výsledek na webu

<a href="https://doi.org/10.1039/D3AN00046J" target="_blank" >https://doi.org/10.1039/D3AN00046J</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d3an00046j" target="_blank" >10.1039/d3an00046j</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells

Popis výsledku v původním jazyce

Single-benzene fluorophores are bright and the smallest fluorochromes known so far. In single-benzene fluorophores, the fluorescence is mediated by the push/pull effect of substituting groups. Despite a plethora of advantageous properties, this group of molecules has not been extensively studied for design of high-performance fluorescent sensors of catalytic or enzymatic activities. Thus, herein, new fluorescent probes based on the Tsuji-Trost reaction were developed for the selective detection of palladium and other transition metals (platinum and gold) in an aqueous/organic mixed solvent with the sensitivity down to 2.5 nM (for palladium). The relative flexibility in the synthesis of these probes allows for facile color tuning of the emitted fluorescence. In this study, we have successfully utilized a yellow emission variant for sensitive detection of palladium under cell-free conditions and in living cells, validating its possible applicability for high-throughput optical sensing of catalysts for bioorthogonal chemistry under physiological conditions.

Název v anglickém jazyce

A color-tunable single-benzene fluorophore-based sensor for sensitive detection of palladium in solution and living cells

Popis výsledku anglicky

Single-benzene fluorophores are bright and the smallest fluorochromes known so far. In single-benzene fluorophores, the fluorescence is mediated by the push/pull effect of substituting groups. Despite a plethora of advantageous properties, this group of molecules has not been extensively studied for design of high-performance fluorescent sensors of catalytic or enzymatic activities. Thus, herein, new fluorescent probes based on the Tsuji-Trost reaction were developed for the selective detection of palladium and other transition metals (platinum and gold) in an aqueous/organic mixed solvent with the sensitivity down to 2.5 nM (for palladium). The relative flexibility in the synthesis of these probes allows for facile color tuning of the emitted fluorescence. In this study, we have successfully utilized a yellow emission variant for sensitive detection of palladium under cell-free conditions and in living cells, validating its possible applicability for high-throughput optical sensing of catalysts for bioorthogonal chemistry under physiological conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analyst

ISSN

0003-2654

e-ISSN

1364-5528

Svazek periodika

148

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

2058-2063

Kód UT WoS článku

000959188700001

EID výsledku v databázi Scopus

2-s2.0-85151833491