Polycaprolactone fiber-based bovine dendritic cell development and three- dimensional culture in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43923810" target="_blank" >RIV/62156489:43210/23:43923810 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.55251/jmbfs.10304" target="_blank" >https://doi.org/10.55251/jmbfs.10304</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.55251/jmbfs.10304" target="_blank" >10.55251/jmbfs.10304</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polycaprolactone fiber-based bovine dendritic cell development and three- dimensional culture in vitro

Popis výsledku v původním jazyce

Two-dimensional (2D) cell culture systems are normally used for in vitro experiments. These systems have some drawbacks which affect results of in vitro experiments because cultured cells have different morphology and physiological properties comparing to in vivo models. Therefore, there are current technique to develop three-dimensional (3D) cell culture systems which are closer to the environment in real tissues. In our study, we used polycaprolactone fiber scaffold for culture of bovine monocytes to develop dendritic cells (DCs) from those cells. Cultured monocytes in the polycaprolactone nanofiber scaffold differentiated to DCs. The differentiated DCs showed a 3D structure in the scaffold. These results suggest that this method is suitable for the development of DCs in a manner of three-dimension. These methods are perspective for bovine cell culture and has advantages compare to conventional 2D culture systems.

Název v anglickém jazyce

Polycaprolactone fiber-based bovine dendritic cell development and three- dimensional culture in vitro

Popis výsledku anglicky

Two-dimensional (2D) cell culture systems are normally used for in vitro experiments. These systems have some drawbacks which affect results of in vitro experiments because cultured cells have different morphology and physiological properties comparing to in vivo models. Therefore, there are current technique to develop three-dimensional (3D) cell culture systems which are closer to the environment in real tissues. In our study, we used polycaprolactone fiber scaffold for culture of bovine monocytes to develop dendritic cells (DCs) from those cells. Cultured monocytes in the polycaprolactone nanofiber scaffold differentiated to DCs. The differentiated DCs showed a 3D structure in the scaffold. These results suggest that this method is suitable for the development of DCs in a manner of three-dimension. These methods are perspective for bovine cell culture and has advantages compare to conventional 2D culture systems.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

1338-5178

Svazek periodika

13

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

4

Strana od-do

"e10304"

Kód UT WoS článku

001021634100001

EID výsledku v databázi Scopus

2-s2.0-85173787188