Functionalization of 3D Fibrous Scaffolds Prepared Using Centrifugal Spinning with Liposomes as a Simple Drug Delivery System

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21720%2F17%3A00317968" target="_blank" >RIV/68407700:21720/17:00317968 - isvavai.cz</a>

Výsledek na webu

<a href="http://ojs.cvut.cz/ojs/index.php/ap" target="_blank" >http://ojs.cvut.cz/ojs/index.php/ap</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14311/APP.2017.8.0024" target="_blank" >10.14311/APP.2017.8.0024</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Functionalization of 3D Fibrous Scaffolds Prepared Using Centrifugal Spinning with Liposomes as a Simple Drug Delivery System

Popis výsledku v původním jazyce

3D materials supporting cell adhesion, infiltration and proliferation are crucial for bone tissue engineering. To fulfill these expectations, it is beneficial to combine the 3D scaffold with drug delivery systems. Release of the bioactive molecules directly in the site of the defect results in attraction of cells and their proliferation and differentiation in the required cell type. Only properly differentiated cells produce proteins of extracellular matrix and can form new tissue. In the current study, we propose a simple drug delivery system composed of polycaprolactone fibers, prepared using centrifugal spinning, with adhered liposomes filled with platelet lysate. Platelets contain wide range of growth factors and cytokines and can be used as their natural and autologous source. The scaffold was tested in vitro using MG-63 osteoblast-like cells. Cell adhesion, proliferation and osteogenic differentiation were determined. Three different concentrations of liposomes were used and compared to a scaffold with control liposomes filled only with PBS and to the plain polycaprolactone fibers. The results of the study showed that the platelet lysate stimulated cell proliferation. On the other hand, its effect on osteogenic differentiation was not proved. The combination of polymeric fibers with liposomes via simple adhesion was shown to be a promising scaffold for bone tissue engineering applications. In the future, it is necessary to focus on the stability of liposomes to prevent the burst release of the incorporated molecules.

Název v anglickém jazyce

Functionalization of 3D Fibrous Scaffolds Prepared Using Centrifugal Spinning with Liposomes as a Simple Drug Delivery System

Popis výsledku anglicky

3D materials supporting cell adhesion, infiltration and proliferation are crucial for bone tissue engineering. To fulfill these expectations, it is beneficial to combine the 3D scaffold with drug delivery systems. Release of the bioactive molecules directly in the site of the defect results in attraction of cells and their proliferation and differentiation in the required cell type. Only properly differentiated cells produce proteins of extracellular matrix and can form new tissue. In the current study, we propose a simple drug delivery system composed of polycaprolactone fibers, prepared using centrifugal spinning, with adhered liposomes filled with platelet lysate. Platelets contain wide range of growth factors and cytokines and can be used as their natural and autologous source. The scaffold was tested in vitro using MG-63 osteoblast-like cells. Cell adhesion, proliferation and osteogenic differentiation were determined. Three different concentrations of liposomes were used and compared to a scaffold with control liposomes filled only with PBS and to the plain polycaprolactone fibers. The results of the study showed that the platelet lysate stimulated cell proliferation. On the other hand, its effect on osteogenic differentiation was not proved. The combination of polymeric fibers with liposomes via simple adhesion was shown to be a promising scaffold for bone tissue engineering applications. In the future, it is necessary to focus on the stability of liposomes to prevent the burst release of the incorporated molecules.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30500 - Other medical sciences

Projekt

<a href="/cs/project/GA15-15697S" target="_blank" >GA15-15697S: Třídimenzionální nanovlákenný nosič s inkorporovaným systémem řízeného dodávaní léčiv pro tkáńové inženýrství kosti a chrupavky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

Název statě ve sborníku

Acta Polytechnica CTU Proceedings: BIOMATERIALS AND SURFACES 2016

ISBN

978-80-01-06135-0

ISSN

2336-5382

e-ISSN

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Počet stran výsledku

3

Strana od-do

24-26

Název nakladatele

Czech Technical University in Prague

Místo vydání

Praha

Místo konání akce

Vyšší Brod, Herbertov

Datum konání akce

20. 9. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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