Needleless electrospun and centrifugal spun poly-epsilon-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00518445" target="_blank" >RIV/68378041:_____/19:00518445 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21220/19:00327459 RIV/68407700:21720/19:00327459 RIV/00216208:11310/19:10394293 RIV/00216208:11130/19:10394293

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0928493118302625?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0928493118302625?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2018.12.069" target="_blank" >10.1016/j.msec.2018.12.069</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Needleless electrospun and centrifugal spun poly-epsilon-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs

Popis výsledku v původním jazyce

The biofunctionalization of scaffolds for tissue engineering is crucial to improve the results of regenerative therapies. This study compared the effect of platelet-functionalization of 2D electrospun and 3D centrifugal spun scaffolds on the osteogenic potential of hMSCs. Scaffolds prepared from poly-epsilon-caprolactone, using electrospinning and centrifugal spinning technology, were functionalized using five different concentrations of platelets. Cell proliferation, metabolic activity and osteogenic differentiation were tested using hMSCs cultured in differential and non-differential medium. The porous 3D structure of the centrifugal spun fibers resulted in higher cell proliferation. Furthermore, the functionalization of the scaffolds with platelets resulted in a dose dependent increase in cell metabolic activity, proliferation and production of an osteogenic marker alkaline phosphatase. The effect was further promoted by culture in an osteogenic differential medium. The increase in combination of both platelets and osteogenic media shows an improved osteoinduction by platelets in environments rich in inorganic phosphate and ascorbate. Nevertheless, the results of the study showed that the optimal concentration of platelets for induction of hMSC osteogenesis is in the range of 900-3000 x 10(9) platelets/L. The study determines the potential of electrospun and centrifugal spun fibers with adhered platelets, for use in bone tissue engineering.

Název v anglickém jazyce

Needleless electrospun and centrifugal spun poly-epsilon-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs

Popis výsledku anglicky

The biofunctionalization of scaffolds for tissue engineering is crucial to improve the results of regenerative therapies. This study compared the effect of platelet-functionalization of 2D electrospun and 3D centrifugal spun scaffolds on the osteogenic potential of hMSCs. Scaffolds prepared from poly-epsilon-caprolactone, using electrospinning and centrifugal spinning technology, were functionalized using five different concentrations of platelets. Cell proliferation, metabolic activity and osteogenic differentiation were tested using hMSCs cultured in differential and non-differential medium. The porous 3D structure of the centrifugal spun fibers resulted in higher cell proliferation. Furthermore, the functionalization of the scaffolds with platelets resulted in a dose dependent increase in cell metabolic activity, proliferation and production of an osteogenic marker alkaline phosphatase. The effect was further promoted by culture in an osteogenic differential medium. The increase in combination of both platelets and osteogenic media shows an improved osteoinduction by platelets in environments rich in inorganic phosphate and ascorbate. Nevertheless, the results of the study showed that the optimal concentration of platelets for induction of hMSC osteogenesis is in the range of 900-3000 x 10(9) platelets/L. The study determines the potential of electrospun and centrifugal spun fibers with adhered platelets, for use in bone tissue engineering.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Science & Engineering C-Materials for Biological Applications

ISSN

0928-4931

e-ISSN

—

Svazek periodika

97

Číslo periodika v rámci svazku

apr.

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

567-575

Kód UT WoS článku

000457952800054

EID výsledku v databázi Scopus

2-s2.0-85059050644