Exogenously applied putrescine and chitosan–putrescine nanocomposite alleviate the negative effects of chilling stress on iceberg lettuce seedlings

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F25%3A43927355" target="_blank" >RIV/62156489:43210/25:43927355 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1038/s41598-025-11180-x" target="_blank" >https://doi.org/10.1038/s41598-025-11180-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-11180-x" target="_blank" >10.1038/s41598-025-11180-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exogenously applied putrescine and chitosan–putrescine nanocomposite alleviate the negative effects of chilling stress on iceberg lettuce seedlings

Popis výsledku v původním jazyce

Climate change-induced stressors such as extreme temperatures negatively affect plant growth and development. Therefore, methods are needed to improve plant tolerance to abiotic stresses. The aim of this study was to evaluate whether the foliar application of putrescine (Put) and a chitosan-putrescine nanocomposite (Ch-Put) can modulate the antioxidant response and membrane properties of lettuce under chilling stress. In this study, Put and Ch-Put were applied via foliar spraying at two concentrations (1 mM and 2.5 mM), and after treatment, the plants were placed in a phytotron at 4 oC and 20 oC. Changes in the properties of the cell membranes were assessed in the seedlings. Additionally, the antioxidant enzymatic activity and content of nonenzymatic bioactive compounds were evaluated. The results indicated that the use of Put and Ch-Put influenced the permeability and fluidity of the lipid membranes, which also depended on the treatment temperature. An increase in Alim (all treatments) and a decrease in Cs-1 values (Put and Ch-Put at a concentration of 2.5 mM) were observed at 4 oC, suggesting looser packing and increased elasticity of cell membranes, facilitating the metabolic and physiological adaptation of plants to stress. The treatment of chilled plants with Put and Ch-Put resulted in increased contents of proline, carbohydrates, glutathione, phenolics and L-ascorbic acid, as well as increased activity of several antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), which could contribute to increased tolerance to stress. The strongest effects were observed for Put at concentrations of 1 mM and 2.5 mM and Ch-Put at the 2.5 mM concentration, suggesting the potential use of these substances in strategies to increase plant tolerance to chilling stress.

Název v anglickém jazyce

Exogenously applied putrescine and chitosan–putrescine nanocomposite alleviate the negative effects of chilling stress on iceberg lettuce seedlings

Popis výsledku anglicky

Climate change-induced stressors such as extreme temperatures negatively affect plant growth and development. Therefore, methods are needed to improve plant tolerance to abiotic stresses. The aim of this study was to evaluate whether the foliar application of putrescine (Put) and a chitosan-putrescine nanocomposite (Ch-Put) can modulate the antioxidant response and membrane properties of lettuce under chilling stress. In this study, Put and Ch-Put were applied via foliar spraying at two concentrations (1 mM and 2.5 mM), and after treatment, the plants were placed in a phytotron at 4 oC and 20 oC. Changes in the properties of the cell membranes were assessed in the seedlings. Additionally, the antioxidant enzymatic activity and content of nonenzymatic bioactive compounds were evaluated. The results indicated that the use of Put and Ch-Put influenced the permeability and fluidity of the lipid membranes, which also depended on the treatment temperature. An increase in Alim (all treatments) and a decrease in Cs-1 values (Put and Ch-Put at a concentration of 2.5 mM) were observed at 4 oC, suggesting looser packing and increased elasticity of cell membranes, facilitating the metabolic and physiological adaptation of plants to stress. The treatment of chilled plants with Put and Ch-Put resulted in increased contents of proline, carbohydrates, glutathione, phenolics and L-ascorbic acid, as well as increased activity of several antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), which could contribute to increased tolerance to stress. The strongest effects were observed for Put at concentrations of 1 mM and 2.5 mM and Ch-Put at the 2.5 mM concentration, suggesting the potential use of these substances in strategies to increase plant tolerance to chilling stress.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

15

Číslo periodika v rámci svazku

24 July

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

20

Strana od-do

26963

Kód UT WoS článku

001537443500013

EID výsledku v databázi Scopus

2-s2.0-105011539149