ZnO nanoparticle effects on Arabidopsis thaliana: Insights into miRNA and metabolite profiles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F25%3A43927564" target="_blank" >RIV/62156489:43210/25:43927564 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00142956

Výsledek na webu

<a href="https://doi.org/10.1016/j.genrep.2025.102352" target="_blank" >https://doi.org/10.1016/j.genrep.2025.102352</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.genrep.2025.102352" target="_blank" >10.1016/j.genrep.2025.102352</a>

Jazyk výsledku

angličtina

Název v původním jazyce

ZnO nanoparticle effects on Arabidopsis thaliana: Insights into miRNA and metabolite profiles

Popis výsledku v původním jazyce

The application of zinc oxide nanoparticles (ZnO NPs) in agriculture and plant research is a topic of significant debate. In this study, we explored the molecular responses of Arabidopsis thaliana (Columbia (Col-0) ecotype) to ZnO NP exposure, with a primary focus on miRNA expression and metabolite profiles, providing new insights into the mechanisms underlying plant responses. ZnO NPs were applied at three concentrations (8, 40, 80 mg.kgMINUS SIGN 1) in peat tablets (Jiffy(R)). Our results reveal dose-dependent negative effects on plant phenotypic traits, particularly reductions in growth and chlorophyll content. Notably, ZnO NPs caused significant disruptions in metal homeostasis, with elevated zinc accumulation and altered uptake of manganese, iron, and calcium. Gene expression analysis of antioxidant defense responses demonstrated concentration-specific regulation, indicating a shift towards more specialized antioxidants under ZnO NP stress. Specifically, the lower concentrations (8 and 40 mg.kgMINUS SIGN 1) led to the overexpression of genes related to H2O2 scavenging and glutathione synthesis, while the 80 mg.kgMINUS SIGN 1 concentration induced the upregulation of genes associated with superoxide scavenging, highlighting a dose-dependent shift in oxidative stress response mechanisms. These molecular changes were accompanied by significant alterations in miRNA expression and metabolite profiles, in a non-linear and dose-dependent manner. A significant role was observed for the miR156-SPL regulatory module at the 40 and 80 mg.kgMINUS SIGN 1 concentrations. Furthermore, proline (approx. 20 % increase, 8, 80 mg.kgMINUS SIGN 1), taurine (16 %, 80 mg.kgMINUS SIGN 1), fumaric acid (31 %, 40 mg.kgMINUS SIGN 1), and glutamic acid (50 %, 8 mg.kgMINUS SIGN 1) were implicated in the plant&apos;s adaptive response to ZnO NPs, contributing to antioxidant defense and metal-binding mechanisms. Together, these results highlight that ZnO NP exposure elicits complex and multi-layered adaptive mechanisms involving the interplay of elemental stress, antioxidant responses, miRNA regulation, and metabolic adjustments.

Název v anglickém jazyce

ZnO nanoparticle effects on Arabidopsis thaliana: Insights into miRNA and metabolite profiles

Popis výsledku anglicky

The application of zinc oxide nanoparticles (ZnO NPs) in agriculture and plant research is a topic of significant debate. In this study, we explored the molecular responses of Arabidopsis thaliana (Columbia (Col-0) ecotype) to ZnO NP exposure, with a primary focus on miRNA expression and metabolite profiles, providing new insights into the mechanisms underlying plant responses. ZnO NPs were applied at three concentrations (8, 40, 80 mg.kgMINUS SIGN 1) in peat tablets (Jiffy(R)). Our results reveal dose-dependent negative effects on plant phenotypic traits, particularly reductions in growth and chlorophyll content. Notably, ZnO NPs caused significant disruptions in metal homeostasis, with elevated zinc accumulation and altered uptake of manganese, iron, and calcium. Gene expression analysis of antioxidant defense responses demonstrated concentration-specific regulation, indicating a shift towards more specialized antioxidants under ZnO NP stress. Specifically, the lower concentrations (8 and 40 mg.kgMINUS SIGN 1) led to the overexpression of genes related to H2O2 scavenging and glutathione synthesis, while the 80 mg.kgMINUS SIGN 1 concentration induced the upregulation of genes associated with superoxide scavenging, highlighting a dose-dependent shift in oxidative stress response mechanisms. These molecular changes were accompanied by significant alterations in miRNA expression and metabolite profiles, in a non-linear and dose-dependent manner. A significant role was observed for the miR156-SPL regulatory module at the 40 and 80 mg.kgMINUS SIGN 1 concentrations. Furthermore, proline (approx. 20 % increase, 8, 80 mg.kgMINUS SIGN 1), taurine (16 %, 80 mg.kgMINUS SIGN 1), fumaric acid (31 %, 40 mg.kgMINUS SIGN 1), and glutamic acid (50 %, 8 mg.kgMINUS SIGN 1) were implicated in the plant&apos;s adaptive response to ZnO NPs, contributing to antioxidant defense and metal-binding mechanisms. Together, these results highlight that ZnO NP exposure elicits complex and multi-layered adaptive mechanisms involving the interplay of elemental stress, antioxidant responses, miRNA regulation, and metabolic adjustments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Gene Reports

ISSN

2452-0144

e-ISSN

2452-0144

Svazek periodika

41

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

102352

Kód UT WoS článku

001600047800001

EID výsledku v databázi Scopus

2-s2.0-105018504804