Supercritical Fluid Extraction of Bio-Available Amino Acids in Soils and their Liquid Chromatographic Determination with Fluorimetric Detection.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F06%3A00094584" target="_blank" >RIV/62156489:43410/06:00094584 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Supercritical Fluid Extraction of Bio-Available Amino Acids in Soils and their Liquid Chromatographic Determination with Fluorimetric Detection

Popis výsledku v původním jazyce

A new procedure with supercritical CO2 modified with 0.5 ml water and 0.75 ml 0.1 M HCl in situ and 0.75 ml of water on-line (7.5 % v/v) at 15 MPa, 50 °C, for 45 min was applied for extraction of bio-available amino acids from soil samples. Total extraction time was 60 min but more favourable conditions are even possible for selected groups of amino acids. All analytes were trapped into 20 ml of MeOH with satisfying recovery (94 % - 104 %) and determined using high-performance liquid chromatography withfluorimetric detection (HPLC/FLD) on Zorbax Eclipse column (4.6 x 75 mm, 3.5 micrometers) with Na2HPO4 and ACN:MeOH:water as a mobile phase. Linear calibration curves were obtained (r is higher than 0.999 except 0.99823 for Ile) with lower limits of detection in the range of 1.54 pg (Gly) - 13.45 pg (Cy2), or 18.6 fmol (Ser) - 64.8 fmol (Lys). HPLC validation and repeatability data are also given. Comparable results were obtained with a robust, commonly used extraction method (0.5 M amm

Název v anglickém jazyce

Supercritical Fluid Extraction of Bio-Available Amino Acids in Soils and their Liquid Chromatographic Determination with Fluorimetric Detection

Popis výsledku anglicky

A new procedure with supercritical CO2 modified with 0.5 ml water and 0.75 ml 0.1 M HCl in situ and 0.75 ml of water on-line (7.5 % v/v) at 15 MPa, 50 °C, for 45 min was applied for extraction of bio-available amino acids from soil samples. Total extraction time was 60 min but more favourable conditions are even possible for selected groups of amino acids. All analytes were trapped into 20 ml of MeOH with satisfying recovery (94 % - 104 %) and determined using high-performance liquid chromatography withfluorimetric detection (HPLC/FLD) on Zorbax Eclipse column (4.6 x 75 mm, 3.5 micrometers) with Na2HPO4 and ACN:MeOH:water as a mobile phase. Linear calibration curves were obtained (r is higher than 0.999 except 0.99823 for Ile) with lower limits of detection in the range of 1.54 pg (Gly) - 13.45 pg (Cy2), or 18.6 fmol (Ser) - 64.8 fmol (Lys). HPLC validation and repeatability data are also given. Comparable results were obtained with a robust, commonly used extraction method (0.5 M amm

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GP526%2F03%2FD058" target="_blank" >GP526/03/D058: Biologicky přístupné aminokyseliny v půdě pod lučními společenstvy Moravskoslezských Beskyd</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

6130-6138

Kód UT WoS článku

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EID výsledku v databázi Scopus

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