Aktivita pudnich fosfataz v pudach luznich lesu
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F07%3A00109782" target="_blank" >RIV/62156489:43410/07:00109782 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Acid phosphomonoesterase activity in floodplain forest soils
Popis výsledku v původním jazyce
The 4-nitrophenyl phosphate method for acid phosphomonoesterase (EC 3.1.3.2; acid orthophosphoric monoester phosphohydrolase) determination was slightly modified to increase the sensitivity and the stability of the 4-nitrophenol coloured complex in samples rich in humic substances and easily soluble organic compounds. Based on an approach used for mycorrhizal roots, the new analytical protocol was tested on samples taken from a single forest site with a large variation in soil types. Distinctive properties of forest soils and the accuracy/repeatability of the optimised technique were considered in the selection of the most appropriate analytical steps. Finally, the acid phosphomonoesterase activity was expressed in micrograms of 4-nitrophenol/g fresh soil recalculated dry matter/h released after hydrolysis of 4-nitrophenyl phosphate in sodium tetraborate/succinic acid buffer at pH 4.8. The modified method proposed was statistically compared with other procedures and the results obtaine
Název v anglickém jazyce
Acid phosphomonoesterase activity in floodplain forest soils
Popis výsledku anglicky
The 4-nitrophenyl phosphate method for acid phosphomonoesterase (EC 3.1.3.2; acid orthophosphoric monoester phosphohydrolase) determination was slightly modified to increase the sensitivity and the stability of the 4-nitrophenol coloured complex in samples rich in humic substances and easily soluble organic compounds. Based on an approach used for mycorrhizal roots, the new analytical protocol was tested on samples taken from a single forest site with a large variation in soil types. Distinctive properties of forest soils and the accuracy/repeatability of the optimised technique were considered in the selection of the most appropriate analytical steps. Finally, the acid phosphomonoesterase activity was expressed in micrograms of 4-nitrophenol/g fresh soil recalculated dry matter/h released after hydrolysis of 4-nitrophenyl phosphate in sodium tetraborate/succinic acid buffer at pH 4.8. The modified method proposed was statistically compared with other procedures and the results obtaine
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
DF - Pedologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2007
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Soil and Water Research
ISSN
1801-5395
e-ISSN
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Svazek periodika
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Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
9
Strana od-do
67-75
Kód UT WoS článku
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EID výsledku v databázi Scopus
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