Multiple Viral Infections Detected in Phytophthora condilina by Total and Small RNA Sequencing
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F21%3A43919463" target="_blank" >RIV/62156489:43410/21:43919463 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.3390/v13040620" target="_blank" >https://doi.org/10.3390/v13040620</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/v13040620" target="_blank" >10.3390/v13040620</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Multiple Viral Infections Detected in Phytophthora condilina by Total and Small RNA Sequencing
Popis výsledku v původním jazyce
Marine oomycetes have recently been shown to be concurrently infected by (MINUS SIGN )ssRNA viruses of the order Bunyavirales. In this work, even higher virus variability was found in a single isolate of Phytophthora condilina, a recently described member of Phytophthora phylogenetic Clade 6a, which was isolated from brackish estuarine waters in southern Portugal. Using total and small RNA-seq the full RdRp of 13 different potential novel bunya-like viruses and two complete toti-like viruses were detected. All these viruses were successfully confirmed by reverse transcription polymerase chain reaction (RT-PCR) using total RNA as template, but complementarily one of the toti-like and five of the bunya-like viruses were confirmed when dsRNA was purified for RT-PCR. In our study, total RNA-seq was by far more efficient for de novo assembling of the virus sequencing but small RNA-seq showed higher read numbers for most viruses. Two main populations of small RNAs (21 nts and 25 nts-long) were identified, which were in accordance with other Phytophthora species. To the best of our knowledge, this is the first study using small RNA sequencing to identify viruses in Phytophthora spp.
Název v anglickém jazyce
Multiple Viral Infections Detected in Phytophthora condilina by Total and Small RNA Sequencing
Popis výsledku anglicky
Marine oomycetes have recently been shown to be concurrently infected by (MINUS SIGN )ssRNA viruses of the order Bunyavirales. In this work, even higher virus variability was found in a single isolate of Phytophthora condilina, a recently described member of Phytophthora phylogenetic Clade 6a, which was isolated from brackish estuarine waters in southern Portugal. Using total and small RNA-seq the full RdRp of 13 different potential novel bunya-like viruses and two complete toti-like viruses were detected. All these viruses were successfully confirmed by reverse transcription polymerase chain reaction (RT-PCR) using total RNA as template, but complementarily one of the toti-like and five of the bunya-like viruses were confirmed when dsRNA was purified for RT-PCR. In our study, total RNA-seq was by far more efficient for de novo assembling of the virus sequencing but small RNA-seq showed higher read numbers for most viruses. Two main populations of small RNAs (21 nts and 25 nts-long) were identified, which were in accordance with other Phytophthora species. To the best of our knowledge, this is the first study using small RNA sequencing to identify viruses in Phytophthora spp.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10607 - Virology
Návaznosti výsledku
Projekt
<a href="/cs/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Výzkumné centrum pro studium patogenů z rodu Phytophthora</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Viruses
ISSN
1999-4915
e-ISSN
—
Svazek periodika
13
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
18
Strana od-do
620
Kód UT WoS článku
000643802200001
EID výsledku v databázi Scopus
2-s2.0-85105195930