Natural Populations from the Phytophthora palustris Complex Show a High Diversity and Abundance of ssRNA and dsRNA Viruses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F22%3A43922471" target="_blank" >RIV/62156489:43410/22:43922471 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3390/jof8111118" target="_blank" >https://doi.org/10.3390/jof8111118</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/jof8111118" target="_blank" >10.3390/jof8111118</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Natural Populations from the Phytophthora palustris Complex Show a High Diversity and Abundance of ssRNA and dsRNA Viruses

Popis výsledku v původním jazyce

We explored the virome of the &quot;Phytophthora palustris complex&quot;, a group of aquatic specialists geographically limited to Southeast and East Asia, the native origin of many destructive invasive forest Phytophthora spp. Based on high-throughput sequencing (RNAseq) of 112 isolates of &quot;P. palustris&quot; collected from rivers, mangroves, and ponds, and natural forests in subtropical and tropical areas in Indonesia, Taiwan, and Japan, 52 putative viruses were identified, which, to varying degrees, were phylogenetically related to the families Botybirnaviridae, Narnaviridae, Tombusviridae, and Totiviridae, and the order Bunyavirales. The prevalence of all viruses in their hosts was investigated and confirmed by RT-PCR. The rich virus composition, high abundance, and distribution discovered in our study indicate that viruses are naturally infecting taxa from the &quot;P. palustris complex&quot; in their natural niche, and that they are predominant members of the host cellular environment. Certain Indonesian localities are the viruses&apos; hotspots and particular &quot;P. palustris&quot; isolates show complex multiviral infections. This study defines the first bi-segmented bunya-like virus together with the first tombus-like and botybirna-like viruses in the genus Phytophthora and provides insights into the spread and evolution of RNA viruses in the natural populations of an oomycete species.

Název v anglickém jazyce

Natural Populations from the Phytophthora palustris Complex Show a High Diversity and Abundance of ssRNA and dsRNA Viruses

Popis výsledku anglicky

We explored the virome of the &quot;Phytophthora palustris complex&quot;, a group of aquatic specialists geographically limited to Southeast and East Asia, the native origin of many destructive invasive forest Phytophthora spp. Based on high-throughput sequencing (RNAseq) of 112 isolates of &quot;P. palustris&quot; collected from rivers, mangroves, and ponds, and natural forests in subtropical and tropical areas in Indonesia, Taiwan, and Japan, 52 putative viruses were identified, which, to varying degrees, were phylogenetically related to the families Botybirnaviridae, Narnaviridae, Tombusviridae, and Totiviridae, and the order Bunyavirales. The prevalence of all viruses in their hosts was investigated and confirmed by RT-PCR. The rich virus composition, high abundance, and distribution discovered in our study indicate that viruses are naturally infecting taxa from the &quot;P. palustris complex&quot; in their natural niche, and that they are predominant members of the host cellular environment. Certain Indonesian localities are the viruses&apos; hotspots and particular &quot;P. palustris&quot; isolates show complex multiviral infections. This study defines the first bi-segmented bunya-like virus together with the first tombus-like and botybirna-like viruses in the genus Phytophthora and provides insights into the spread and evolution of RNA viruses in the natural populations of an oomycete species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

<a href="/cs/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Výzkumné centrum pro studium patogenů z rodu Phytophthora</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Fungi

ISSN

2309-608X

e-ISSN

2309-608X

Svazek periodika

8

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

28

Strana od-do

1118

Kód UT WoS článku

000882185100001

EID výsledku v databázi Scopus

2-s2.0-85141752701