Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F09%3A00170204" target="_blank" >RIV/62156489:43510/09:00170204 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis

Popis výsledku v původním jazyce

The aim of the work was to develop the preparation of ds cDNA from flower buds of four apricot varieties ('Sundrop', 'Stark Early Orange', 'Vestar' and 'Betinka') so that it would be as suitable as possible for the cDNA-AFLP analysis. The sampled tissueswere preserved using a commercial agent RNAlater Soln. (Ambion). The RNA from the chosen samples was isolated using a commercial isolation kit supplied by Sigma (SpectrumTM Plant Total RNA Kit), which support the isolation of sufficient amounts of highquality RNA from these highly complicated tissues. Transcription from the entire RNA onto the ds cDNA was achieved using the MINT cDNA synthesis kit from Evrogen. The cDNA-AFLP analysis was carried out according to an amended procedure by BACHEM et al. (1998) and the samples obtained from selective amplification were analysed on genetic analyser ABI-PRISM 310. The results obtained by another protocol (include silica extraction of RNA, GenElute mRNA Miniprep Kit and others) were compared

Název v anglickém jazyce

Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis

Popis výsledku anglicky

The aim of the work was to develop the preparation of ds cDNA from flower buds of four apricot varieties ('Sundrop', 'Stark Early Orange', 'Vestar' and 'Betinka') so that it would be as suitable as possible for the cDNA-AFLP analysis. The sampled tissueswere preserved using a commercial agent RNAlater Soln. (Ambion). The RNA from the chosen samples was isolated using a commercial isolation kit supplied by Sigma (SpectrumTM Plant Total RNA Kit), which support the isolation of sufficient amounts of highquality RNA from these highly complicated tissues. Transcription from the entire RNA onto the ds cDNA was achieved using the MINT cDNA synthesis kit from Evrogen. The cDNA-AFLP analysis was carried out according to an amended procedure by BACHEM et al. (1998) and the samples obtained from selective amplification were analysed on genetic analyser ABI-PRISM 310. The results obtained by another protocol (include silica extraction of RNA, GenElute mRNA Miniprep Kit and others) were compared

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta horticulturae

ISSN

0567-7572

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

839

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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