Utility of retrotransposon-derived marker systems for differentiation of presumed clones of the apricot cultivar Velkopavlovická

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F12%3A00198168" target="_blank" >RIV/62156489:43510/12:00198168 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utility of retrotransposon-derived marker systems for differentiation of presumed clones of the apricot cultivar Velkopavlovická

Popis výsledku v původním jazyce

Apricot cultivars may be composed of a variety of clones differing in minor genetic and phenotypic characteristics. Transposable elements might be considered, among other factors, a potential source of mutations that lead to clonal variation. Therefore,genetic markers derived from transposable regions should be suitable for differentiation of clones. The aims of the present work were to verify the utility of sequence-specific amplified polymorphism (SSAP) markers and inter-primer binding sites (iPBS) as universal retrotransposon-derived marker systems for differentiation of clones on the basis of their different DNA fingerprints and to compare the utility of SSAP and iPBS systems with other, more commonly used marker systems (SSR and AFLP). The results obtained with SSAP and iPBS marker systems were similar to those with the conventional AFLP method. Thus, the hypothesis that these retrotransposon-based markers should be more suitable for clonal differentiation was not confirmed. An a

Název v anglickém jazyce

Utility of retrotransposon-derived marker systems for differentiation of presumed clones of the apricot cultivar Velkopavlovická

Popis výsledku anglicky

Apricot cultivars may be composed of a variety of clones differing in minor genetic and phenotypic characteristics. Transposable elements might be considered, among other factors, a potential source of mutations that lead to clonal variation. Therefore,genetic markers derived from transposable regions should be suitable for differentiation of clones. The aims of the present work were to verify the utility of sequence-specific amplified polymorphism (SSAP) markers and inter-primer binding sites (iPBS) as universal retrotransposon-derived marker systems for differentiation of clones on the basis of their different DNA fingerprints and to compare the utility of SSAP and iPBS systems with other, more commonly used marker systems (SSR and AFLP). The results obtained with SSAP and iPBS marker systems were similar to those with the conventional AFLP method. Thus, the hypothesis that these retrotransposon-based markers should be more suitable for clonal differentiation was not confirmed. An a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Horticulturae

ISSN

0304-4238

e-ISSN

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Svazek periodika

143

Číslo periodika v rámci svazku

143

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

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EID výsledku v databázi Scopus

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