The effect of media composition on multiplication of grape rootstocks in vitro
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F12%3A43908951" target="_blank" >RIV/62156489:43510/12:43908951 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.11118/actaun201260080141" target="_blank" >http://dx.doi.org/10.11118/actaun201260080141</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.11118/actaun201260080141" target="_blank" >10.11118/actaun201260080141</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The effect of media composition on multiplication of grape rootstocks in vitro
Popis výsledku v původním jazyce
The current demand for in vitro cultures of grape rootstocks, not only for mass production of plants, but also for genetic engineering is evident. The study on micropropagation of grape rootstock genotypes namely Kober 5BB, Kober 125AA and Teleki 5C was performed. The aim of the study was to develop an optimized protocol to obtain large quantity of plant material. Protocol is based on regeneration via organogenesis, considering that grape embryogenic calluses are laborious to establish and the genotype of the regenerated plants can be altered. Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks. Subsequent cultivation on modified Murashige Skoog media with 1-naphtalene acetic acid and increased concentration of cytokynin was characterized by multiplication of cultures and formation of clusters with high multiplication capability. The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.
Název v anglickém jazyce
The effect of media composition on multiplication of grape rootstocks in vitro
Popis výsledku anglicky
The current demand for in vitro cultures of grape rootstocks, not only for mass production of plants, but also for genetic engineering is evident. The study on micropropagation of grape rootstock genotypes namely Kober 5BB, Kober 125AA and Teleki 5C was performed. The aim of the study was to develop an optimized protocol to obtain large quantity of plant material. Protocol is based on regeneration via organogenesis, considering that grape embryogenic calluses are laborious to establish and the genotype of the regenerated plants can be altered. Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks. Subsequent cultivation on modified Murashige Skoog media with 1-naphtalene acetic acid and increased concentration of cytokynin was characterized by multiplication of cultures and formation of clusters with high multiplication capability. The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GE - Šlechtění rostlin
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/QH91214" target="_blank" >QH91214: Nové biotechnologické postupy pro navození rezistence podnoží révy vinné proti nepovirům</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta universitatis Agriculturae et Silviculturae Mendelianae Brunensis
ISSN
1211-8516
e-ISSN
—
Svazek periodika
60
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
4
Strana od-do
141-144
Kód UT WoS článku
—
EID výsledku v databázi Scopus
2-s2.0-84874104721