Evaluation of Three Approaches For The Measurement of Rna Integrity, Concentration and Purity in Tissues of Apricot Flower Buds
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F14%3A00214101" target="_blank" >RIV/62156489:43510/14:00214101 - isvavai.cz</a>
Výsledek na webu
<a href="http://theglobaljournals.com/ijsr/issues.php?m=March&y=2014&id=23" target="_blank" >http://theglobaljournals.com/ijsr/issues.php?m=March&y=2014&id=23</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Evaluation of Three Approaches For The Measurement of Rna Integrity, Concentration and Purity in Tissues of Apricot Flower Buds
Popis výsledku v původním jazyce
The RNA quantity and quality are basic factors for successful studies of gene expression. On absorbance and fluorescence based measurements are routinely used techniques for RNA measurement. A new technique for measurement of RNA is available with Agilent 2100 bioanalyzer in combination with the RNA 6000 Nano LabChip kit. This method offers in addition the advantage of assessing RNA integrity. Agilent 2100 Bioanalyzer is compared with UV spectrophotometry and fluorometry quantitation systems assessing the quantity, purity and RNA integrity. This study was focused on comparison of RNA isolated from apricot flower buds which were evaluated by mentioned methods. Obtained results show important differences in RNA which was isolated in 2008 (stored at -80 C) and RNA which was newly isolated from the same stored tissues in 2013. Study also compares techniques of mRNA quantity, quality and integrity evaluation.
Název v anglickém jazyce
Evaluation of Three Approaches For The Measurement of Rna Integrity, Concentration and Purity in Tissues of Apricot Flower Buds
Popis výsledku anglicky
The RNA quantity and quality are basic factors for successful studies of gene expression. On absorbance and fluorescence based measurements are routinely used techniques for RNA measurement. A new technique for measurement of RNA is available with Agilent 2100 bioanalyzer in combination with the RNA 6000 Nano LabChip kit. This method offers in addition the advantage of assessing RNA integrity. Agilent 2100 Bioanalyzer is compared with UV spectrophotometry and fluorometry quantitation systems assessing the quantity, purity and RNA integrity. This study was focused on comparison of RNA isolated from apricot flower buds which were evaluated by mentioned methods. Obtained results show important differences in RNA which was isolated in 2008 (stored at -80 C) and RNA which was newly isolated from the same stored tissues in 2013. Study also compares techniques of mRNA quantity, quality and integrity evaluation.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EI - Biotechnologie a bionika
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International Journal of Scientific Research
ISSN
2277-8179
e-ISSN
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Svazek periodika
3
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
IN - Indická republika
Počet stran výsledku
4
Strana od-do
157-160
Kód UT WoS článku
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EID výsledku v databázi Scopus
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