Relative expression of gammadelta T-cell receptor gene families detected by RT?PCR and capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F12%3A00059919" target="_blank" >RIV/00216224:14330/12:00059919 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/12:#0000957

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1744-313X.2012.01114.x/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1744-313X.2012.01114.x/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1744-313X.2012.01114.x" target="_blank" >10.1111/j.1744-313X.2012.01114.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Relative expression of gammadelta T-cell receptor gene families detected by RT?PCR and capillary electrophoresis

Popis výsledku v původním jazyce

Gammadelta T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the gamma variable (Vgamma) and delta variable (Vdelta) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region (CDR3) spectratyping, but a fast and simple assessment of Vgamma and Vdelta gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the gammadelta T-cell population. This report provides optimized PCR conditions for gammadelta T-cell receptor amplification. Further it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of Vgamma and Vdelta gene families after their amplification.

Název v anglickém jazyce

Relative expression of gammadelta T-cell receptor gene families detected by RT?PCR and capillary electrophoresis

Popis výsledku anglicky

Gammadelta T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post-transplant period is not yet fully understood. PCR-based techniques were established to study the gamma variable (Vgamma) and delta variable (Vdelta) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity-determining region (CDR3) spectratyping, but a fast and simple assessment of Vgamma and Vdelta gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the gammadelta T-cell population. This report provides optimized PCR conditions for gammadelta T-cell receptor amplification. Further it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of Vgamma and Vdelta gene families after their amplification.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of immunogenetics

ISSN

1744-3121

e-ISSN

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Svazek periodika

Epub

Číslo periodika v rámci svazku

2012 Apr10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1-8

Kód UT WoS článku

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EID výsledku v databázi Scopus

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