Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F10%3A00045361" target="_blank" >RIV/00216224:14330/10:00045361 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire

Popis výsledku v původním jazyce

Monitoring the T-cell population diversity has demonstrated to be beneficial when evaluating an individuals immune status. BV families are most often analysed to examine this diversity. The aim of our study was to establish a method for specific determination of relative BV family expression and to evaluate its applicability in terms of multiple myeloma (MM). Peripheral blood mononuclear cells from healthy donors and MM patients were analysed by RT-PCR. PCR product amounts were determined with an Agilent 2100 Bioanalyzer utilizing capillary electrophoresis and converted to the relative BV family expression. This report provides reverse transcription PCR with primers allowing for the analysis of the entire BV family repertoire. Further it describes theutilization of capillary electrophoresis to evaluate the relative BV family expression after their amplification. The methodology is fast and simple to operate and is therefore suitable as a first screening of the T-cell population compos

Název v anglickém jazyce

Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire

Popis výsledku anglicky

Monitoring the T-cell population diversity has demonstrated to be beneficial when evaluating an individuals immune status. BV families are most often analysed to examine this diversity. The aim of our study was to establish a method for specific determination of relative BV family expression and to evaluate its applicability in terms of multiple myeloma (MM). Peripheral blood mononuclear cells from healthy donors and MM patients were analysed by RT-PCR. PCR product amounts were determined with an Agilent 2100 Bioanalyzer utilizing capillary electrophoresis and converted to the relative BV family expression. This report provides reverse transcription PCR with primers allowing for the analysis of the entire BV family repertoire. Further it describes theutilization of capillary electrophoresis to evaluate the relative BV family expression after their amplification. The methodology is fast and simple to operate and is therefore suitable as a first screening of the T-cell population compos

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/2B06052" target="_blank" >2B06052: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Advances in Molecular and Cancer Biology, 2010

ISBN

978-80-210-5312-0

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

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Název nakladatele

muni PRESS

Místo vydání

Brno, Česká republika

Místo konání akce

Brno, Česká Republika

Datum konání akce

11. 11. 2010

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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