Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F11%3A%230001582" target="_blank" >RIV/65269705:_____/11:#0001582 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14330/11:00051519

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.clim.2010.09.007" target="_blank" >http://dx.doi.org/10.1016/j.clim.2010.09.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.clim.2010.09.007" target="_blank" >10.1016/j.clim.2010.09.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families

Popis výsledku v původním jazyce

The immune systems of multiple myeloma patients are suppressed by the disease itself, and this immunosuppression is further enhanced by standard therapies. The aim of our study was to evaluate the effects of initial chemotherapy and a peripheral blood mobilisation regimen on T-cell population diversity. Reverse transcription polymerase chain reaction (RT-PCR) with a new set of primers, in combination with capillary electrophoresis, was established. The methodology was used to analyse the relative expression of 27 T-cell receptor beta variable gene families (BV families) in multiple myeloma patients undergoing peripheral blood stem cell harvest. We found that the overall BV family usage in these patients was restricted; the relative expression of 10 BVfamilies was significantly depressed in patients compared to healthy donors. These findings demonstrate that the preparative regimen for autologous stem cell transplantation affects the T-cell population in terms of the restriction of its

Název v anglickém jazyce

Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families

Popis výsledku anglicky

The immune systems of multiple myeloma patients are suppressed by the disease itself, and this immunosuppression is further enhanced by standard therapies. The aim of our study was to evaluate the effects of initial chemotherapy and a peripheral blood mobilisation regimen on T-cell population diversity. Reverse transcription polymerase chain reaction (RT-PCR) with a new set of primers, in combination with capillary electrophoresis, was established. The methodology was used to analyse the relative expression of 27 T-cell receptor beta variable gene families (BV families) in multiple myeloma patients undergoing peripheral blood stem cell harvest. We found that the overall BV family usage in these patients was restricted; the relative expression of 10 BVfamilies was significantly depressed in patients compared to healthy donors. These findings demonstrate that the preparative regimen for autologous stem cell transplantation affects the T-cell population in terms of the restriction of its

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Immunology

ISSN

1521-6616

e-ISSN

—

Svazek periodika

138

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

67-76

Kód UT WoS článku

000286714000010

EID výsledku v databázi Scopus

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