Use of real-time PCR for the characterization of variable symptoms in ESFY phytoplasma disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F16%3A43908417" target="_blank" >RIV/62156489:43510/16:43908417 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1110.9" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2016.1110.9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1110.9" target="_blank" >10.17660/ActaHortic.2016.1110.9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Use of real-time PCR for the characterization of variable symptoms in ESFY phytoplasma disease

Popis výsledku v původním jazyce

'Candidatus phytoplasma prunorum', known as ESFY phytoplasma, is the agent of apricot and peach illness, with very variable symptoms. Symptom variability depends on cultivar, type of rootstock and especially on virulence and concentration of the pathogen. In this experiment a long term monitored set of 12 trees was selected for their symptoms variability and indicated by ESFY phytoplasma. The aim of the work was to find the concentration of phytoplasmatic DNA at 24 samples (taken from leaf petioles and from phloem) and finding correlation between type and intensity of symptomatic effect and concentration. The results clearly show that the concentration of pathogenic DNA is 1.36x higher in herbaceous parts - petioles - than in phloem. Generally, the highest concentration was detected in the samples taken from the trees with symptoms of chlorotic leaf roll, average 1.6x105 copies μL-1 in the samples taken from phloem and 2.1x105 copies μL-1 in the samples taken from petioles. The lowest concentration was in the samples from the tree with symptoms of leaf roll beginning infection ESFY). The sample taken from the tree with latent infection had a 22.7% lower concentration of phytoplasmatic DNA than average sample (in the case of DNA isolated from petiole). Results also show that in case of the 'Poyer' tree cultivar with latent infection there may be an infection with hypovirulent strains or it is a tolerant genotype to ESFY phytoplasma.

Název v anglickém jazyce

Use of real-time PCR for the characterization of variable symptoms in ESFY phytoplasma disease

Popis výsledku anglicky

'Candidatus phytoplasma prunorum', known as ESFY phytoplasma, is the agent of apricot and peach illness, with very variable symptoms. Symptom variability depends on cultivar, type of rootstock and especially on virulence and concentration of the pathogen. In this experiment a long term monitored set of 12 trees was selected for their symptoms variability and indicated by ESFY phytoplasma. The aim of the work was to find the concentration of phytoplasmatic DNA at 24 samples (taken from leaf petioles and from phloem) and finding correlation between type and intensity of symptomatic effect and concentration. The results clearly show that the concentration of pathogenic DNA is 1.36x higher in herbaceous parts - petioles - than in phloem. Generally, the highest concentration was detected in the samples taken from the trees with symptoms of chlorotic leaf roll, average 1.6x105 copies μL-1 in the samples taken from phloem and 2.1x105 copies μL-1 in the samples taken from petioles. The lowest concentration was in the samples from the tree with symptoms of leaf roll beginning infection ESFY). The sample taken from the tree with latent infection had a 22.7% lower concentration of phytoplasmatic DNA than average sample (in the case of DNA isolated from petiole). Results also show that in case of the 'Poyer' tree cultivar with latent infection there may be an infection with hypovirulent strains or it is a tolerant genotype to ESFY phytoplasma.

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae 1110

ISBN

978-94-6261-104-7

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

6

Strana od-do

57-62

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Brisbane

Datum konání akce

17. 8. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000391249900009