Determination of synergistic interactions between Plum pox virus and 'Candidatus Phytoplasma prunorum' in infected peach trees
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43912335" target="_blank" >RIV/62156489:43510/17:43912335 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1163.8" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2017.1163.8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1163.8" target="_blank" >10.17660/ActaHortic.2017.1163.8</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of synergistic interactions between Plum pox virus and 'Candidatus Phytoplasma prunorum' in infected peach trees
Popis výsledku v původním jazyce
This assessment of the interaction between Plum pox virus (PPV) and European stone fruit yellows (ESFY; caused by 'Candidatus Phytoplasma prunorum') infections in two peach cultivars, 'Symphony' and 'Royal Glory', took place in the years 2012-2013. Trees showing symptoms of PPV were artificially infected with ESFY and, conversely, trees with ESFY were infected with PPV (the D strain). Trees infected with only one of the two pathogens acted as controls. Synergistic and antagonistic interactions were visually assessed using a four-point scoring system for the principal symptoms characteristic of each pathogen, accompanied by quantitative measurements of the pathogenic organisms present in leaf tissue (using nested PCR and measuring the titre in DAS-ELISA). PPV-infected trees inoculated with ESFY showed reduced levels of PPV symptoms, but ESFY-infected trees inoculated with PPV displayed enhanced symptoms of both infections, in what might be called a synergistic manner. Laboratory measurements showed that the leaves of ESFY-inoculated trees had average relative concentrations of PPV of 3.3x10-1 ('Symphony') and 2.5x10-1 ('Royal Glory') and average relative concentrations of the ESFY phytoplasma of 24,303 copies μL-1 DNA ('Symphony') and 268,000 copies μL-1 DNA ('Royal Glory'). The leaves of PPV-inoculated trees had an average relative concentration of PPV of 4.4x10-1 and an average concentration of ESFY phytoplasma of 32,849 copies μL-1 DNA.
Název v anglickém jazyce
Determination of synergistic interactions between Plum pox virus and 'Candidatus Phytoplasma prunorum' in infected peach trees
Popis výsledku anglicky
This assessment of the interaction between Plum pox virus (PPV) and European stone fruit yellows (ESFY; caused by 'Candidatus Phytoplasma prunorum') infections in two peach cultivars, 'Symphony' and 'Royal Glory', took place in the years 2012-2013. Trees showing symptoms of PPV were artificially infected with ESFY and, conversely, trees with ESFY were infected with PPV (the D strain). Trees infected with only one of the two pathogens acted as controls. Synergistic and antagonistic interactions were visually assessed using a four-point scoring system for the principal symptoms characteristic of each pathogen, accompanied by quantitative measurements of the pathogenic organisms present in leaf tissue (using nested PCR and measuring the titre in DAS-ELISA). PPV-infected trees inoculated with ESFY showed reduced levels of PPV symptoms, but ESFY-infected trees inoculated with PPV displayed enhanced symptoms of both infections, in what might be called a synergistic manner. Laboratory measurements showed that the leaves of ESFY-inoculated trees had average relative concentrations of PPV of 3.3x10-1 ('Symphony') and 2.5x10-1 ('Royal Glory') and average relative concentrations of the ESFY phytoplasma of 24,303 copies μL-1 DNA ('Symphony') and 268,000 copies μL-1 DNA ('Royal Glory'). The leaves of PPV-inoculated trees had an average relative concentration of PPV of 4.4x10-1 and an average concentration of ESFY phytoplasma of 32,849 copies μL-1 DNA.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
—
OECD FORD obor
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Návaznosti výsledku
Projekt
<a href="/cs/project/QJ1510352" target="_blank" >QJ1510352: Hodnocení faktorů ovlivňujících škodlivost fytoplazem napadajících ovocné dřeviny a ověřování účinných prostředků jejich eliminace</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Acta Horticulturae 1163
ISBN
978-94-6261-158-0
ISSN
0567-7572
e-ISSN
2406-6168
Počet stran výsledku
8
Strana od-do
45-52
Název nakladatele
International Society for Horticultural Science
Místo vydání
Leuven
Místo konání akce
Antalya
Datum konání akce
9. 5. 2016
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000428232700008