Impact of Anions, Cations, and pH on Manganese Accumulation and Toxicity in the Green Alga Scenedesmus quadricauda

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F16%3A43909561" target="_blank" >RIV/62156489:43510/16:43909561 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/16:00090098

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11270-016-2859-2" target="_blank" >http://dx.doi.org/10.1007/s11270-016-2859-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11270-016-2859-2" target="_blank" >10.1007/s11270-016-2859-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of Anions, Cations, and pH on Manganese Accumulation and Toxicity in the Green Alga Scenedesmus quadricauda

Popis výsledku v původním jazyce

Accumulation of divalent manganese (Mn) and its toxicity in the green alga Scenedesmus quadricauda was studied at circumneutral pH (6.5). A comparison of two applied concentrations (10 or 100 mu M) of MnCl2, MnSO4, and Mn(NO3)(2) indicated that mainly sulfate evoked higher Mn accumulation. On the other hand, nitrate rather depleted antioxidative enzyme activities (APX, CAT, SOD), leading to an increase in ROS formation as proven by fluorescence microscopy. Subsequent experiments revealed that increase in pH (from 4.5 to 9.5) increased also Mn content but typically depleted amounts of reduced glutathione and phytochelatin 2. We also measured the size of particles formed from the manganese salts at pH 9.5. Competitive experiment between Ca/K salts (CaCl2, CaSO4, Ca(NO3)(2), KCl, K2SO4, KNO3) and Mn (as Mn sulfate) showed a negative relation between Ca and Mn amount but KNO3 stimulated Mn accumulation. Microscopy revealed that mainly K salts elevated plasma membrane damage (Acridine orange staining). Data indicate that not only pH but also accompanying anion affects Mn accumulation and that Ca salts may affect Mn toxicity.

Název v anglickém jazyce

Impact of Anions, Cations, and pH on Manganese Accumulation and Toxicity in the Green Alga Scenedesmus quadricauda

Popis výsledku anglicky

Accumulation of divalent manganese (Mn) and its toxicity in the green alga Scenedesmus quadricauda was studied at circumneutral pH (6.5). A comparison of two applied concentrations (10 or 100 mu M) of MnCl2, MnSO4, and Mn(NO3)(2) indicated that mainly sulfate evoked higher Mn accumulation. On the other hand, nitrate rather depleted antioxidative enzyme activities (APX, CAT, SOD), leading to an increase in ROS formation as proven by fluorescence microscopy. Subsequent experiments revealed that increase in pH (from 4.5 to 9.5) increased also Mn content but typically depleted amounts of reduced glutathione and phytochelatin 2. We also measured the size of particles formed from the manganese salts at pH 9.5. Competitive experiment between Ca/K salts (CaCl2, CaSO4, Ca(NO3)(2), KCl, K2SO4, KNO3) and Mn (as Mn sulfate) showed a negative relation between Ca and Mn amount but KNO3 stimulated Mn accumulation. Microscopy revealed that mainly K salts elevated plasma membrane damage (Acridine orange staining). Data indicate that not only pH but also accompanying anion affects Mn accumulation and that Ca salts may affect Mn toxicity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/EE2.3.30.0017" target="_blank" >EE2.3.30.0017: Postdoktorandi v oborech biologických věd na MENDELU</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Water, Air, and Soil Pollution

ISSN

0049-6979

e-ISSN

—

Svazek periodika

227

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

000376276100032

EID výsledku v databázi Scopus

2-s2.0-84964786214