Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F16%3A43909732" target="_blank" >RIV/62156489:43510/16:43909732 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2298/GENSR1602629K" target="_blank" >http://dx.doi.org/10.2298/GENSR1602629K</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2298/GENSR1602629K" target="_blank" >10.2298/GENSR1602629K</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas

Popis výsledku v původním jazyce

In this work, two real-time PCR protocols based on intercalating dye and two on hydrolysis probes were tested using field collected fruit tree samples infected by 16SrX group (AP, PD and ESFY) phytoplasmas. Specificity and sensitivity of protocols and amplification efficiency were the main testing parameters. Results of real-time PCR protocols were compared to nested PCR. All real-time PCR protocols confirmed their specificity of detection. All real-time PCR protocols were 10-100 times more sensitive than nested PCR. Afterall real-time PCR protocols based on hydrolysis probes were 10 times more sensitive than protocols based on intercalating dyes. Among protocols based on hydrolysis probes, slightly better detection characteristics were shown by protocol by CHRISTENSEN et al. (2004).

Název v anglickém jazyce

Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas

Popis výsledku anglicky

In this work, two real-time PCR protocols based on intercalating dye and two on hydrolysis probes were tested using field collected fruit tree samples infected by 16SrX group (AP, PD and ESFY) phytoplasmas. Specificity and sensitivity of protocols and amplification efficiency were the main testing parameters. Results of real-time PCR protocols were compared to nested PCR. All real-time PCR protocols confirmed their specificity of detection. All real-time PCR protocols were 10-100 times more sensitive than nested PCR. Afterall real-time PCR protocols based on hydrolysis probes were 10 times more sensitive than protocols based on intercalating dyes. Among protocols based on hydrolysis probes, slightly better detection characteristics were shown by protocol by CHRISTENSEN et al. (2004).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

—

Projekt

<a href="/cs/project/QJ1510352" target="_blank" >QJ1510352: Hodnocení faktorů ovlivňujících škodlivost fytoplazem napadajících ovocné dřeviny a ověřování účinných prostředků jejich eliminace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetika

ISSN

0534-0012

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

14

Strana od-do

629-642

Kód UT WoS článku

000389658100016

EID výsledku v databázi Scopus

2-s2.0-84994517482