SIMULTANEOUS DETECTION OF PEACH AND APRICOT DNA BY MULTIPLEX REAL-TIME PCR-HRM WITH INTERCALATING DYE

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

SIMULTANEOUS DETECTION OF PEACH AND APRICOT DNA BY MULTIPLEX REAL-TIME PCR-HRM WITH INTERCALATING DYE

Popis výsledku v původním jazyce

Multiplex real-time PCR-HRM with specific primers and intercalating dye was suggested as a new method for authentication of products containing buffalo meat by Sakaridis et al [1]. It has since been used for authentication of other meat products and medicinal plant products, but not for authentication of fruit-based foodstuffs. In this work we tested this method with DNA isolated from peach and apricot fruits and with two primer pairs, whose specifity was verified by real-time PCR‐HRM [2, 3]. The multiplex real‐time PCR‐HRM was carried out with equal amounts of DNA from both fruit species, and its sensitivity was tested with decreasing amounts of each DNA (1 ng, 100 pg, 10 pg, 1 pg). We detected peach DNA in assays with 1 ng to 10 pg of DNA, and apricot DNA was detected in assays with 1 ng to 100 pg of DNA. We were able to detect two disctinct amplicones in all assays in which both peach and apricot DNA was detected.

Název v anglickém jazyce

SIMULTANEOUS DETECTION OF PEACH AND APRICOT DNA BY MULTIPLEX REAL-TIME PCR-HRM WITH INTERCALATING DYE

Popis výsledku anglicky

Multiplex real-time PCR-HRM with specific primers and intercalating dye was suggested as a new method for authentication of products containing buffalo meat by Sakaridis et al [1]. It has since been used for authentication of other meat products and medicinal plant products, but not for authentication of fruit-based foodstuffs. In this work we tested this method with DNA isolated from peach and apricot fruits and with two primer pairs, whose specifity was verified by real-time PCR‐HRM [2, 3]. The multiplex real‐time PCR‐HRM was carried out with equal amounts of DNA from both fruit species, and its sensitivity was tested with decreasing amounts of each DNA (1 ng, 100 pg, 10 pg, 1 pg). We detected peach DNA in assays with 1 ng to 10 pg of DNA, and apricot DNA was detected in assays with 1 ng to 100 pg of DNA. We were able to detect two disctinct amplicones in all assays in which both peach and apricot DNA was detected.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů