Development of a Multiplex PCR Assay for Simultaneous Detection of Closely Related Fruit Species in Food

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU142712" target="_blank" >RIV/00216305:26310/21:PU142712 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of a Multiplex PCR Assay for Simultaneous Detection of Closely Related Fruit Species in Food

Popis výsledku v původním jazyce

Food adulteration is a long-standing problem. As new ways of adulteration develop, new methods for food authenticity checking, including DNA-based ones, should be developed, too. In this work we focused on multiplex real-time PCR-HRM and its possible use in simultaneous detection of various fruit species in food. New apricot-specific primers were also designed as a part of this work. Peach and apricot were chosen as the fruit species of interest because they occur as both the adulterant and the adulterated commodity [1], and because certain foodstuffs (such as infant food)contain both of these fruits. We analyzed three fruit purees, two of which contained approximately 70 % of apple and 30 % of either apricot or peach. In these purees, neither apricot nor peach was detected. The third puree contained both apricot and peach, but not apple. Using multiplex PCR, we were able to detect apricot in the last puree by both HRM analysis and electrophoresis, and peach was detected by electrophoresis. From thes

Název v anglickém jazyce

Development of a Multiplex PCR Assay for Simultaneous Detection of Closely Related Fruit Species in Food

Popis výsledku anglicky

Food adulteration is a long-standing problem. As new ways of adulteration develop, new methods for food authenticity checking, including DNA-based ones, should be developed, too. In this work we focused on multiplex real-time PCR-HRM and its possible use in simultaneous detection of various fruit species in food. New apricot-specific primers were also designed as a part of this work. Peach and apricot were chosen as the fruit species of interest because they occur as both the adulterant and the adulterated commodity [1], and because certain foodstuffs (such as infant food)contain both of these fruits. We analyzed three fruit purees, two of which contained approximately 70 % of apple and 30 % of either apricot or peach. In these purees, neither apricot nor peach was detected. The third puree contained both apricot and peach, but not apple. Using multiplex PCR, we were able to detect apricot in the last puree by both HRM analysis and electrophoresis, and peach was detected by electrophoresis. From thes

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů