First report of Little cherry virus 1 infecting apricot in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43912336" target="_blank" >RIV/62156489:43510/17:43912336 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1094/PDIS-09-16-1289-PDN" target="_blank" >http://dx.doi.org/10.1094/PDIS-09-16-1289-PDN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1094/PDIS-09-16-1289-PDN" target="_blank" >10.1094/PDIS-09-16-1289-PDN</a>

Jazyk výsledku

angličtina

Název v původním jazyce

First report of Little cherry virus 1 infecting apricot in the Czech Republic

Popis výsledku v původním jazyce

It was first described in 1996, is believed to be widely distributed in cherry and, together with Little cherry virus 2, is associated with the little cherry disease. It is listed as a quarantine agent in various countries. Various cherry species, but mainly sour and sweet cherry, are known to be host plants, although recently almond, peach, and plum trees infected by LChV-1 were reported. During a survey, apricots (Prunus armeniaca L.), representing 21 different cultivars, were sampled in a germplasm collection in Lednice, Czech Republic, in early summer 2016. In PCR assay using the LChV-1 specific primers LCV1U16390 and LCV1L16809 targeting an ORF8 fragment), a product of the expected 419 bp size was obtained from five apricot cultivars: Velkopavlovicka VP-LE-12, VP-LE-118, Magiar Kajszii, Madarska, and Marlen. The amplicons were sequenced directly (GenBank accession nos. KX831094-99) and their estimated divergence based on the maximum composite likelihood model varied between 0.001 and 0.023 nucleotide substitutions per site. The Czech isolates are homologous in the sequenced fragment to the ITMAR isolate showing 92.6 to 95.7% nucleotide identity with this European LChV-1 isolate. The presence of LChV-1 in the five positive apricot cultivars was confirmed by using an independent RT-PCR assay, which targets a different genomic region (CP gene) and uses the primers 1LC_12776F and 1LC_13223R described by. The expected 449-bp PCR fragment was obtained from all apricots found positive in the first RT-PCR screening. Although the presence of LChV-1 had been previously reported on cherry in the Czech Republic, as far as we know, this is the first report of LChV-1 infecting apricots, thus identifying P. armeniaca as a new host of this virus. The importance of apricots as a host for LChV-1, given its relatively high frequency in the tested germplasm collection but the absence of clear symptomatology, remains to be evaluated in detail.

Název v anglickém jazyce

First report of Little cherry virus 1 infecting apricot in the Czech Republic

Popis výsledku anglicky

It was first described in 1996, is believed to be widely distributed in cherry and, together with Little cherry virus 2, is associated with the little cherry disease. It is listed as a quarantine agent in various countries. Various cherry species, but mainly sour and sweet cherry, are known to be host plants, although recently almond, peach, and plum trees infected by LChV-1 were reported. During a survey, apricots (Prunus armeniaca L.), representing 21 different cultivars, were sampled in a germplasm collection in Lednice, Czech Republic, in early summer 2016. In PCR assay using the LChV-1 specific primers LCV1U16390 and LCV1L16809 targeting an ORF8 fragment), a product of the expected 419 bp size was obtained from five apricot cultivars: Velkopavlovicka VP-LE-12, VP-LE-118, Magiar Kajszii, Madarska, and Marlen. The amplicons were sequenced directly (GenBank accession nos. KX831094-99) and their estimated divergence based on the maximum composite likelihood model varied between 0.001 and 0.023 nucleotide substitutions per site. The Czech isolates are homologous in the sequenced fragment to the ITMAR isolate showing 92.6 to 95.7% nucleotide identity with this European LChV-1 isolate. The presence of LChV-1 in the five positive apricot cultivars was confirmed by using an independent RT-PCR assay, which targets a different genomic region (CP gene) and uses the primers 1LC_12776F and 1LC_13223R described by. The expected 449-bp PCR fragment was obtained from all apricots found positive in the first RT-PCR screening. Although the presence of LChV-1 had been previously reported on cherry in the Czech Republic, as far as we know, this is the first report of LChV-1 infecting apricots, thus identifying P. armeniaca as a new host of this virus. The importance of apricots as a host for LChV-1, given its relatively high frequency in the tested germplasm collection but the absence of clear symptomatology, remains to be evaluated in detail.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Disease

ISSN

0191-2917

e-ISSN

—

Svazek periodika

101

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

1

Strana od-do

845

Kód UT WoS článku

000399860900065

EID výsledku v databázi Scopus

2-s2.0-85017517618