MicroRNAs in Vitis vinifera cv. Chardonnay are differentially expressed in response to Diaporthe species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43916626" target="_blank" >RIV/62156489:43510/19:43916626 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3390/genes10110905" target="_blank" >https://doi.org/10.3390/genes10110905</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/genes10110905" target="_blank" >10.3390/genes10110905</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MicroRNAs in Vitis vinifera cv. Chardonnay are differentially expressed in response to Diaporthe species

Popis výsledku v původním jazyce

Diaporthe species are important pathogens, saprobes, and endophytes on grapevines. Several species are known, either as agents of pre-or post-harvest infections, as causal agents of many relevant diseases, including swelling arm, trunk cankers, leaf spots, root and fruit rots, wilts, and cane bleaching. A growing body of evidence exists that a class of small non-coding endogenous RNAs, known as microRNAs (miRNAs), play an important role in post-transcriptional gene regulation, during plant development and responses to biotic and abiotic stresses. In this study, we explored differentially expressed miRNAs in response to Diaporthe eres and Diaporthe bohemiae infection in Vitis vinifera cv. Chardonnay under in vitro conditions. We used computational methods to predict putative miRNA targets in order to explore the involvement of possible pathogen response pathways. We identified 136 known and 41 new miRNA sequence variants, likely generated through post-transcriptional modifications. In the Diaporthe eres treatment, 61 known and 17 new miRNAs were identified while in the Diaporthe bohemiae treatment, 101 known and 21 new miRNAs were revealed. Our results contribute to further understanding the role miRNAs play during plant pathogenesis, which is possibly crucial in understanding disease symptom development in grapevines infected by D. eres and D. bohemiae.

Název v anglickém jazyce

MicroRNAs in Vitis vinifera cv. Chardonnay are differentially expressed in response to Diaporthe species

Popis výsledku anglicky

Diaporthe species are important pathogens, saprobes, and endophytes on grapevines. Several species are known, either as agents of pre-or post-harvest infections, as causal agents of many relevant diseases, including swelling arm, trunk cankers, leaf spots, root and fruit rots, wilts, and cane bleaching. A growing body of evidence exists that a class of small non-coding endogenous RNAs, known as microRNAs (miRNAs), play an important role in post-transcriptional gene regulation, during plant development and responses to biotic and abiotic stresses. In this study, we explored differentially expressed miRNAs in response to Diaporthe eres and Diaporthe bohemiae infection in Vitis vinifera cv. Chardonnay under in vitro conditions. We used computational methods to predict putative miRNA targets in order to explore the involvement of possible pathogen response pathways. We identified 136 known and 41 new miRNA sequence variants, likely generated through post-transcriptional modifications. In the Diaporthe eres treatment, 61 known and 17 new miRNAs were identified while in the Diaporthe bohemiae treatment, 101 known and 21 new miRNAs were revealed. Our results contribute to further understanding the role miRNAs play during plant pathogenesis, which is possibly crucial in understanding disease symptom development in grapevines infected by D. eres and D. bohemiae.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/TJ02000096" target="_blank" >TJ02000096: Metagenomický průzkum zdravotního stavu vinohradů a využití GTD antagononistů čeledi Pseudonocardiaceae</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genes

ISSN

2073-4425

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

19

Strana od-do

905

Kód UT WoS článku

000502296000071

EID výsledku v databázi Scopus

2-s2.0-85074709704