Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F20%3A43918000" target="_blank" >RIV/62156489:43510/20:43918000 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/20:63526154

Výsledek na webu

<a href="https://doi.org/10.3390/agronomy10060868" target="_blank" >https://doi.org/10.3390/agronomy10060868</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy10060868" target="_blank" >10.3390/agronomy10060868</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances

Popis výsledku v původním jazyce

This study aimed to analyze the genetic diversity of selected cultivars and clones of Lonicera sp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity (TAA) in fruits of Lonicera sp. in 2018-2019 was performed. Applied statistical methods evaluating TPC and TAA were: correlation and regression, ANOVA, and agglomerative hierarchical clustering. The neighbor-joining method and gel images analysis (PyElph software, Pavel and Vasile, Bucharest, Romania) were used for genetic analysis. The average TPC of all cultivars and clones was 5.32 mg L-1 in the year 2018 and 3.78 mg L-1 in 2019. The average TAA values of all samples in 2018 were 8.24 mg L-1, and 9.27 mg L-1 in 2019. Statistically significant correlations between years and cultivars and clones were found. Based on the lengths and number of fragments by random amplified polymorphic DNA (RAPD)-PCR&apos;s, it was found that &apos;clone 5&apos; and &apos;clone 7&apos; were genetically close to the grown cultivars from the Czech Republic. Therefore, edible honeysuckle fruits are a valuable source of important nutrients for the human body and its health.

Název v anglickém jazyce

Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances

Popis výsledku anglicky

This study aimed to analyze the genetic diversity of selected cultivars and clones of Lonicera sp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity (TAA) in fruits of Lonicera sp. in 2018-2019 was performed. Applied statistical methods evaluating TPC and TAA were: correlation and regression, ANOVA, and agglomerative hierarchical clustering. The neighbor-joining method and gel images analysis (PyElph software, Pavel and Vasile, Bucharest, Romania) were used for genetic analysis. The average TPC of all cultivars and clones was 5.32 mg L-1 in the year 2018 and 3.78 mg L-1 in 2019. The average TAA values of all samples in 2018 were 8.24 mg L-1, and 9.27 mg L-1 in 2019. Statistically significant correlations between years and cultivars and clones were found. Based on the lengths and number of fragments by random amplified polymorphic DNA (RAPD)-PCR&apos;s, it was found that &apos;clone 5&apos; and &apos;clone 7&apos; were genetically close to the grown cultivars from the Czech Republic. Therefore, edible honeysuckle fruits are a valuable source of important nutrients for the human body and its health.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agronomy

ISSN

2073-4395

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

868

Kód UT WoS článku

000553988500001

EID výsledku v databázi Scopus

2-s2.0-85090667996