Characterization of Tomato Brown Rugose Fruit Virus (ToBRFV) Detected in Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F23%3A43923238" target="_blank" >RIV/62156489:43510/23:43923238 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/23:N0000022

Výsledek na webu

<a href="https://doi.org/10.3390/d15020301" target="_blank" >https://doi.org/10.3390/d15020301</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/d15020301" target="_blank" >10.3390/d15020301</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Tomato Brown Rugose Fruit Virus (ToBRFV) Detected in Czech Republic

Popis výsledku v původním jazyce

Tomato is the most consumed vegetable in the world. The tomato brown rugose fruit virus (ToBRFV) is an important destructive virus that damages tomatoes and peppers with significant economic impact. The detection and characterization of this important viral pathogen were evaluated at the molecular and morphological level. The viral isolate was purified and inoculated on tomato and pepper plants. Small RNAs were sequenced in both plants and the profiles were compared. The complete genome of the isolate was obtained, and microRNA (miRNA) profiles were unveiled by small RNA sequencing. Symptoms caused by the isolate were also described and the morphology of the isolate was observed by transmission electron microscopy. Our results contribute to further understanding of the role of miRNAs in ToBRFV pathogenesis, which may be crucial for understanding disease symptom development in tomatoes and peppers.

Název v anglickém jazyce

Characterization of Tomato Brown Rugose Fruit Virus (ToBRFV) Detected in Czech Republic

Popis výsledku anglicky

Tomato is the most consumed vegetable in the world. The tomato brown rugose fruit virus (ToBRFV) is an important destructive virus that damages tomatoes and peppers with significant economic impact. The detection and characterization of this important viral pathogen were evaluated at the molecular and morphological level. The viral isolate was purified and inoculated on tomato and pepper plants. Small RNAs were sequenced in both plants and the profiles were compared. The complete genome of the isolate was obtained, and microRNA (miRNA) profiles were unveiled by small RNA sequencing. Symptoms caused by the isolate were also described and the morphology of the isolate was observed by transmission electron microscopy. Our results contribute to further understanding of the role of miRNAs in ToBRFV pathogenesis, which may be crucial for understanding disease symptom development in tomatoes and peppers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK22010031" target="_blank" >QK22010031: Využití inovativního potenciálu nanotechnologií pro zvýšení rentability vybraných oblastí zemědělské produkce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diversity

ISSN

1424-2818

e-ISSN

1424-2818

Svazek periodika

15

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

301

Kód UT WoS článku

000938623400001

EID výsledku v databázi Scopus

2-s2.0-85149144868