Caspase Inhibition and N6-Benzyladenosine-lnduced Apoptosis in Hl-60 Cells

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Caspase Inhibition and N6-Benzyladenosine-lnduced Apoptosis in Hl-60 Cells

Popis výsledku v původním jazyce

As an extension of our recently published work (Mlejnek and Kuglík [2000] J. Cell. Biochem. 77:6-17), the role of caspases in N6-benzylaminopurine riboside (BAPR)-induced apotosis in HL-60 cells was evaluated in this study. Here, BAPR-induced apoptosis was accompanied by activation of caspase-3 and caspase-9. However, when these caspases were selectively inhibited, the progression of BAPR-induced apoptosis was not markedly affected. Besides that, activation of caspase-3 and caspase-9 was found to be rather /ate event in apoptotic process. These results suggested that other caspases might be critically implicated. Indeed, pan-specific caspase inhibitor, Z-VAD-FMK completely prevented DNA cleavage and apoptotic bodies formation. However, Z-VAD-FMK failedto prevent CťiL death and it was incapable to fully counteract the main apoptotic hallmark-chromatin condensation.

Název v anglickém jazyce

Caspase Inhibition and N6-Benzyladenosine-lnduced Apoptosis in Hl-60 Cells

Popis výsledku anglicky

As an extension of our recently published work (Mlejnek and Kuglík [2000] J. Cell. Biochem. 77:6-17), the role of caspases in N6-benzylaminopurine riboside (BAPR)-induced apotosis in HL-60 cells was evaluated in this study. Here, BAPR-induced apoptosis was accompanied by activation of caspase-3 and caspase-9. However, when these caspases were selectively inhibited, the progression of BAPR-induced apoptosis was not markedly affected. Besides that, activation of caspase-3 and caspase-9 was found to be rather /ate event in apoptotic process. These results suggested that other caspases might be critically implicated. Indeed, pan-specific caspase inhibitor, Z-VAD-FMK completely prevented DNA cleavage and apoptotic bodies formation. However, Z-VAD-FMK failedto prevent CťiL death and it was incapable to fully counteract the main apoptotic hallmark-chromatin condensation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cellular Biochemistry

ISSN

1097-4644

e-ISSN

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Svazek periodika

83

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

678-689

Kód UT WoS článku

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EID výsledku v databázi Scopus

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