Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F02%3A21100001" target="_blank" >RIV/62156489:_____/02:21100001 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers

Popis výsledku v původním jazyce

Random amplified polymorphic DNA (RAPD) markers were used for the determination of the genetic stability and relationship of clones of Norway spruce (Picea abies (L.) Karst.) early somatic embryo cultures. RAPD analysis of the genetic relationship was carried out on 16 clones of somatic embryos (14 clones derived in our laboratory and two control clones derived in foreign laboratories). Twenty pre-selected primers produced 88 reproducible polymorphic and monomorphic bands, which were evaluated using theRAPDistance Program 1.04. The genetic stability during in vitro culture was studied on 6 clones derived in our laboratory (Brno, Velká Klajdovka) and two control clones. The genomic DNA was isolated in regular intervals 5 times a year during 4 subsequent years. No genetic instability was determined during the 4-year period using 4 selected primers with the highest polymorphisms.

Název v anglickém jazyce

Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers

Popis výsledku anglicky

Random amplified polymorphic DNA (RAPD) markers were used for the determination of the genetic stability and relationship of clones of Norway spruce (Picea abies (L.) Karst.) early somatic embryo cultures. RAPD analysis of the genetic relationship was carried out on 16 clones of somatic embryos (14 clones derived in our laboratory and two control clones derived in foreign laboratories). Twenty pre-selected primers produced 88 reproducible polymorphic and monomorphic bands, which were evaluated using theRAPDistance Program 1.04. The genetic stability during in vitro culture was studied on 6 clones derived in our laboratory (Brno, Velká Klajdovka) and two control clones. The genomic DNA was isolated in regular intervals 5 times a year during 4 subsequent years. No genetic instability was determined during the 4-year period using 4 selected primers with the highest polymorphisms.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologia

ISSN

0006-3088

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

5

Strana od-do

517-521

Kód UT WoS článku

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EID výsledku v databázi Scopus

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