Shigatoxin-producing Escherichia coli O157 in dairy cattle in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F01%3A00000842" target="_blank" >RIV/62157124:16170/01:00000842 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Shigatoxin-producing Escherichia coli O157 in dairy cattle in the Czech Republic

Popis výsledku v původním jazyce

Faecal samples were taken from 715 animals in six dairy cattle herds in the period between January 1997 and October 1999. Samples were examined for E. coli O157 by enrichment in EC broth with novobiocin followed by immunomagnetic separation and culture of concentrated beads onto cefixime tellurite sorbitol McConkey agar. Sorbitol-non-fermenting E.coli O157 isolates were subjected to multiplex PCR analysis for stx1, stx2, hlyA and eae A genes. The overall prevalence of STEC O157 within the set under study was 8.9% (64/715) positive animals in five herds. The distribution of the carriers over the age of dairy cattle was obvious. In the category of calves, heifers and cows respectively 13.0 % (16/123), 21.0% (28/133) and 4.4% (20/459) animals were found positive. All the E.coli O157 strains were stx2, eaeA and hlyA positive. Further characterisation of the STEC O157 strains is under way.

Název v anglickém jazyce

Shigatoxin-producing Escherichia coli O157 in dairy cattle in the Czech Republic

Popis výsledku anglicky

Faecal samples were taken from 715 animals in six dairy cattle herds in the period between January 1997 and October 1999. Samples were examined for E. coli O157 by enrichment in EC broth with novobiocin followed by immunomagnetic separation and culture of concentrated beads onto cefixime tellurite sorbitol McConkey agar. Sorbitol-non-fermenting E.coli O157 isolates were subjected to multiplex PCR analysis for stx1, stx2, hlyA and eae A genes. The overall prevalence of STEC O157 within the set under study was 8.9% (64/715) positive animals in five herds. The distribution of the carriers over the age of dairy cattle was obvious. In the category of calves, heifers and cows respectively 13.0 % (16/123), 21.0% (28/133) and 4.4% (20/459) animals were found positive. All the E.coli O157 strains were stx2, eaeA and hlyA positive. Further characterisation of the STEC O157 strains is under way.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F00%2F0666" target="_blank" >GA525/00/0666: Escherichia coli O157 a další shigatoxigenní sérotypy: identifikace a analýza rizikových faktorů bezpečné produkce potravin na úrovni farmy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

5. Epidemiology of Verocytotoxigenic E.coli

ISBN

1841701475

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

135

Název nakladatele

nezjištěn

Místo vydání

Dublin

Místo konání akce

Ireland

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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