Izolace a charakterizace shigatoxigenních Escherichia coli z ovcí a koz v Jordánsku se zaměřením na multirezistentní sérotyp O157:H7

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F05%3A06G00208" target="_blank" >RIV/00027162:_____/05:06G00208 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7

Popis výsledku v původním jazyce

Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were examined for Shiga toxin- producing E. coli (STEC). The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1 to 3weeksold in one sheep herd with intensive milk production. In remaining herds, serogroups O128, O78, O15 and O128:K85 of STEC strains were most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed byPCR, intwo untypable STEC isolates, stx2 and ehlyA genes. In other STEC non-O157 isolates, only stx1 and ehlyA genes were found. STEC O157:H7 were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicilin andstreptomycin,one agaist co-trimoxazole. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD patern. STEC isolates resistant against antimicrobial agents were demonstrated only in intensive herds.

Název v anglickém jazyce

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats in Jordan with evidence of multiresistant serotype O157:H7

Popis výsledku anglicky

Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were examined for Shiga toxin- producing E. coli (STEC). The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1 to 3weeksold in one sheep herd with intensive milk production. In remaining herds, serogroups O128, O78, O15 and O128:K85 of STEC strains were most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed byPCR, intwo untypable STEC isolates, stx2 and ehlyA genes. In other STEC non-O157 isolates, only stx1 and ehlyA genes were found. STEC O157:H7 were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicilin andstreptomycin,one agaist co-trimoxazole. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD patern. STEC isolates resistant against antimicrobial agents were demonstrated only in intensive herds.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F00%2F0666" target="_blank" >GA525/00/0666: Escherichia coli O157 a další shigatoxigenní sérotypy: identifikace a analýza rizikových faktorů bezpečné produkce potravin na úrovni farmy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

111-118

Kód UT WoS článku

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EID výsledku v databázi Scopus

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