Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F06%3A00000666" target="_blank" >RIV/62157124:16170/06:00000666 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17110/06:A1100VHX

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Popis výsledku v původním jazyce

Out of the total of 153 farms under investigation that had been experiencing diarrhoea in suckling piglets, the presence of Clostridium perfringens was detected on 60 farms (39.2%). PCR typing of isolates prepared by anaerobic cultivation was carried outby multiplex PCR. C. perfringens type A (37.9%) and type C (1.3%) were detected on 58 and 2 farms, respectively. The cpb2 gene was found in 79.3% strains of C. perfringens type A and in 100% strains of type C. An evaluation of the prevalence of positiveidentification of C. perfringens with the cpb2 gene shows that the microorganism was diagnosed on large farms with the basic herd of over 700 or 1 000 sows significantly more frequently. None of the isolates was positive as to the specific sequences ofthe enterotoxin-coding gene.

Název v anglickém jazyce

Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Popis výsledku anglicky

Out of the total of 153 farms under investigation that had been experiencing diarrhoea in suckling piglets, the presence of Clostridium perfringens was detected on 60 farms (39.2%). PCR typing of isolates prepared by anaerobic cultivation was carried outby multiplex PCR. C. perfringens type A (37.9%) and type C (1.3%) were detected on 58 and 2 farms, respectively. The cpb2 gene was found in 79.3% strains of C. perfringens type A and in 100% strains of type C. An evaluation of the prevalence of positiveidentification of C. perfringens with the cpb2 gene shows that the microorganism was diagnosed on large farms with the basic herd of over 700 or 1 000 sows significantly more frequently. None of the isolates was positive as to the specific sequences ofthe enterotoxin-coding gene.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

461-467

Kód UT WoS článku

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EID výsledku v databázi Scopus

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