Effect of two-step vitrification on developmental competence of in vitro and in vivo produces bovine embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F10%3A00003004" target="_blank" >RIV/62157124:16170/10:00003004 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of two-step vitrification on developmental competence of in vitro and in vivo produces bovine embryos

Popis výsledku v původním jazyce

The aim of this study was to establish the effect of two-step vitrification using increasing concentrations of dimethyl sulphoxide and ethylene glycol on survival rate of bovine embryos produced in vitro (method A) and in vivo (method B). In vitro developmental competence of embryos of different quality grade obtained by method B (n = 82) was significantly higher (p < 0.001) compared to method A (n = 98). The best results were detected when we vitrified the embryos of the grade 1 quality. In the case ofdevelopmental competence of embryos at different developmental stages we reached significantly better results (p < 0.001) when we vitrified the embryos produced by method B (n = 84) in comparison with method A (n = 67). We noted a higher hatching rate at the stage of expanded blastocyst; this stage was reached by 7.4% (2/27) of embryos produced by method A and by 30.8% (8/26) of embryos produced by method B (p < 0.05). In total, the hatched blastocyst stage was reached by 15.1% (50/331)

Název v anglickém jazyce

Effect of two-step vitrification on developmental competence of in vitro and in vivo produces bovine embryos

Popis výsledku anglicky

The aim of this study was to establish the effect of two-step vitrification using increasing concentrations of dimethyl sulphoxide and ethylene glycol on survival rate of bovine embryos produced in vitro (method A) and in vivo (method B). In vitro developmental competence of embryos of different quality grade obtained by method B (n = 82) was significantly higher (p < 0.001) compared to method A (n = 98). The best results were detected when we vitrified the embryos of the grade 1 quality. In the case ofdevelopmental competence of embryos at different developmental stages we reached significantly better results (p < 0.001) when we vitrified the embryos produced by method B (n = 84) in comparison with method A (n = 67). We noted a higher hatching rate at the stage of expanded blastocyst; this stage was reached by 7.4% (2/27) of embryos produced by method A and by 30.8% (8/26) of embryos produced by method B (p < 0.05). In total, the hatched blastocyst stage was reached by 15.1% (50/331)

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

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Svazek periodika

79

Číslo periodika v rámci svazku

Suppl. 9

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000282195800009

EID výsledku v databázi Scopus

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