gamadelta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F12%3A43871260" target="_blank" >RIV/62157124:16170/12:43871260 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

gamadelta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra

Popis výsledku v původním jazyce

Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of gamadelta T lymphocytes was found in pyometra samples mainly due to recruitment of gamadelta+/CD8? T lymphocytes. The relative expression of genes encodingselected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1beta, TNF-alfa, IL-8, IL-17 and IFN-gama) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively.

Název v anglickém jazyce

gamadelta T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra

Popis výsledku anglicky

Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of gamadelta T lymphocytes was found in pyometra samples mainly due to recruitment of gamadelta+/CD8? T lymphocytes. The relative expression of genes encodingselected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1beta, TNF-alfa, IL-8, IL-17 and IFN-gama) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

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Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Journal

ISSN

1090-0233

e-ISSN

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Svazek periodika

194

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

303-308

Kód UT WoS článku

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EID výsledku v databázi Scopus

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