Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine herpesvirus 1 and changes caused by serial passage in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F12%3A43871635" target="_blank" >RIV/62157124:16170/12:43871635 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2754/avb201281010009" target="_blank" >http://dx.doi.org/10.2754/avb201281010009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201281010009" target="_blank" >10.2754/avb201281010009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine herpesvirus 1 and changes caused by serial passage in vitro

Popis výsledku v původním jazyce

The aim of this work was to isolate the abortogenic virus strain of equine herpesvirus 1 representing the current infection situation in the Czech Republic, describe it at the molecular level with the accent to genes coding viral glycoproteins and observe the changes caused by passaging of the virus on a cell culture. In 2009, an isolate of equine herpesvirus 1 was obtained from an abortion case in a mare from a herd affected by an abortion storm. The virus identification was performed using the PCR method. The virus was isolated on the RK 13 cell line and after 6 passages in vitro the stability of sequences of selected sections of the virus genome was assessed and compared with the original field isolate. The virus sequences were also compared with known sequences of the abortogenic reference virus strain (V592) and with other known viral strains. One point mutation in a nucleotide sequence coding glycoprotein G was found, distinguishing the field isolate from V592. One point mutation

Název v anglickém jazyce

Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine herpesvirus 1 and changes caused by serial passage in vitro

Popis výsledku anglicky

The aim of this work was to isolate the abortogenic virus strain of equine herpesvirus 1 representing the current infection situation in the Czech Republic, describe it at the molecular level with the accent to genes coding viral glycoproteins and observe the changes caused by passaging of the virus on a cell culture. In 2009, an isolate of equine herpesvirus 1 was obtained from an abortion case in a mare from a herd affected by an abortion storm. The virus identification was performed using the PCR method. The virus was isolated on the RK 13 cell line and after 6 passages in vitro the stability of sequences of selected sections of the virus genome was assessed and compared with the original field isolate. The virus sequences were also compared with known sequences of the abortogenic reference virus strain (V592) and with other known viral strains. One point mutation in a nucleotide sequence coding glycoprotein G was found, distinguishing the field isolate from V592. One point mutation

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/QH92265" target="_blank" >QH92265: Optimalizace systému chovu a veterinární péče o kriticky ohroženou populaci starokladrubského koně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

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Svazek periodika

81

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

9-13

Kód UT WoS článku

000305600200002

EID výsledku v databázi Scopus

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