Dynamics of caspase-3 inhibition in embryonic mouse limbs after developmentally and experimentally induced apoptosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F13%3A43872474" target="_blank" >RIV/62157124:16170/13:43872474 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dynamics of caspase-3 inhibition in embryonic mouse limbs after developmentally and experimentally induced apoptosis

Popis výsledku v původním jazyce

Caspases are key molecules of physiological cell death activated in proteolytic cascade and play a fundamental role during embryonic and postnatal development, particularly in apoptotic events. Caspase-3 is a central caspase of apoptosis, therefore, a common target for manipulations in research and development of anti-cancer therapies. Fluoromethylketones (FMK) are widely used for pharmacological inhibition of caspases, nevertheless, several questions related to inhibitor penetrability, stability and inhibition dynamics remain unclear. Mouse limb digitalization represents the most well known example of apoptosis. This work focuses on caspase-3 inhibition by FMK-inhibitor at the stage prior to the interdigital regression (E12). Two different ex vivo/invitro approaches, explants cultures and mesenchymal micromasses, were exploited to investigate two aspects. The effect of caspase-3 inhibition on limb digitalization was followed using front limb cultures. Inhibition dynamics was evaluate

Název v anglickém jazyce

Dynamics of caspase-3 inhibition in embryonic mouse limbs after developmentally and experimentally induced apoptosis

Popis výsledku anglicky

Caspases are key molecules of physiological cell death activated in proteolytic cascade and play a fundamental role during embryonic and postnatal development, particularly in apoptotic events. Caspase-3 is a central caspase of apoptosis, therefore, a common target for manipulations in research and development of anti-cancer therapies. Fluoromethylketones (FMK) are widely used for pharmacological inhibition of caspases, nevertheless, several questions related to inhibitor penetrability, stability and inhibition dynamics remain unclear. Mouse limb digitalization represents the most well known example of apoptosis. This work focuses on caspase-3 inhibition by FMK-inhibitor at the stage prior to the interdigital regression (E12). Two different ex vivo/invitro approaches, explants cultures and mesenchymal micromasses, were exploited to investigate two aspects. The effect of caspase-3 inhibition on limb digitalization was followed using front limb cultures. Inhibition dynamics was evaluate

Druh

O - Ostatní výsledky

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GAP502%2F12%2F1285" target="_blank" >GAP502/12/1285: Apoptotické a neapoptotické funkce kaspáz při formování tvrdých tkání</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů