Morphological assessment of the reticulocyte maturation in anemic dogs using two different staining methods and the Sysmex XT-2000IV analyzer.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F14%3A43873281" target="_blank" >RIV/62157124:16170/14:43873281 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Morphological assessment of the reticulocyte maturation in anemic dogs using two different staining methods and the Sysmex XT-2000IV analyzer.
Popis výsledku v původním jazyce
Objective: The maturation of reticulocytes is defined by decrease of ribosomal RNA. However its identification using supravital stainings implements important result variability. Parallel analysis on validated Sysmex system recognizing reticulocyte fractions according to the fluorescence intensity may provide more detailed morphological evaluation of erythropoietic activity. Design and Method: The aim of this preliminary study was to assess reticulocyte maturation based on blood smear evaluation using new methylene blue (NMB) and brilliant-cresyl blue (BCB) in 15 anemic dogs. Sysmex XT 2000iV analyzer with subclassification of reticulocytes in low- (LFR), medium- (MFR) and high (HFR) fluorescence reticulocyte fraction was used as a reference method. Reticulocytes in all smears were subdivided in six groups: P1-P3 with punctate pattern (individual distribution or with linear arrangement) and A1-A3 if aggregations were noticed (corresponding to RNA content relative to reticulocyte surfac
Název v anglickém jazyce
Morphological assessment of the reticulocyte maturation in anemic dogs using two different staining methods and the Sysmex XT-2000IV analyzer.
Popis výsledku anglicky
Objective: The maturation of reticulocytes is defined by decrease of ribosomal RNA. However its identification using supravital stainings implements important result variability. Parallel analysis on validated Sysmex system recognizing reticulocyte fractions according to the fluorescence intensity may provide more detailed morphological evaluation of erythropoietic activity. Design and Method: The aim of this preliminary study was to assess reticulocyte maturation based on blood smear evaluation using new methylene blue (NMB) and brilliant-cresyl blue (BCB) in 15 anemic dogs. Sysmex XT 2000iV analyzer with subclassification of reticulocytes in low- (LFR), medium- (MFR) and high (HFR) fluorescence reticulocyte fraction was used as a reference method. Reticulocytes in all smears were subdivided in six groups: P1-P3 with punctate pattern (individual distribution or with linear arrangement) and A1-A3 if aggregations were noticed (corresponding to RNA content relative to reticulocyte surfac
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
GJ - Choroby a škůdci zvířat, veterinární medicina
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů