Single cell analysis of caspase-3 in apoptotic and non-apoptotic cells during mouse limb development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F14%3A43873332" target="_blank" >RIV/62157124:16170/14:43873332 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Single cell analysis of caspase-3 in apoptotic and non-apoptotic cells during mouse limb development

Popis výsledku v původním jazyce

Caspases belong to cysteine proteases participating in apoptosis and inflammation. However, there is recent evidence about their functions also in other events such as cell differentiation. This is supported also by our latest research of odontogenesis and osteogenesis. With the increasing interest in caspases due to their apoptosis-related therapies but also emerging non-apoptotic roles, exact evaluation of their impact at single cell level becomes challenging. We have recently reported about caspase-3detection at femtogram level (10?15 g) in cell populations of embryonic micromasses of chondrogenic mesenchymal cells. To evaluate active caspase-3 in individual cells, a miniaturized device was developed. Such miniaturized device enables quantitation of caspase-3 just in single apoptotic and non-apoptotic cell. The detection is based on the specific cleavage of modified luciferin by caspase-3, bioluminescent emissions of photons and their detection by photomultiplier tube working in th

Název v anglickém jazyce

Single cell analysis of caspase-3 in apoptotic and non-apoptotic cells during mouse limb development

Popis výsledku anglicky

Caspases belong to cysteine proteases participating in apoptosis and inflammation. However, there is recent evidence about their functions also in other events such as cell differentiation. This is supported also by our latest research of odontogenesis and osteogenesis. With the increasing interest in caspases due to their apoptosis-related therapies but also emerging non-apoptotic roles, exact evaluation of their impact at single cell level becomes challenging. We have recently reported about caspase-3detection at femtogram level (10?15 g) in cell populations of embryonic micromasses of chondrogenic mesenchymal cells. To evaluate active caspase-3 in individual cells, a miniaturized device was developed. Such miniaturized device enables quantitation of caspase-3 just in single apoptotic and non-apoptotic cell. The detection is based on the specific cleavage of modified luciferin by caspase-3, bioluminescent emissions of photons and their detection by photomultiplier tube working in th

Druh

O - Ostatní výsledky

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů