BMP Signaling Regulates the Fate of Chondro-osteoprogenitor Cells in Facial Mesenchyme in a Stage-Specific Manner

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F16%3A43874205" target="_blank" >RIV/62157124:16170/16:43874205 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/16:00468150 RIV/00216224:14310/16:00099784

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/dvdy.24422/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/dvdy.24422/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/DVDY.24422" target="_blank" >10.1002/DVDY.24422</a>

Jazyk výsledku

angličtina

Název v původním jazyce

BMP Signaling Regulates the Fate of Chondro-osteoprogenitor Cells in Facial Mesenchyme in a Stage-Specific Manner

Popis výsledku v původním jazyce

Background: Lineage tracing has shown that most of the facial skeleton is derived from cranial neural crest cells. However, the local signals that influence postmigratory, neural crest-derived mesenchyme also play a major role in patterning the skeleton. Here, we study the role of BMP signaling in regulating the fate of chondro-osteoprogenitor cells in the face. Results: A single Noggin-soaked bead inserted into stage 15 chicken embryos induced an ectopic cartilage resembling the interorbital septum within the palate and other midline structures. In contrast, the same treatment in stage 20 embryos caused a loss of bones. The molecular basis for the stage-specific response to Noggin lay in the simultaneous up-regulation of SOX9 and downregulation of RUNX2 in the maxillary mesenchyme, increased cell adhesiveness as shown by N-cadherin induction around the beads and increased RA pathway gene expression. None of these changes were observed in stage 20 embryos. Conclusions: These experiments demonstrate how slight changes in expression of growth factors such as BMPs could lead to gain or loss of cartilage in the upper jaw during vertebrate evolution. In addition, BMPs have at least two roles: one in patterning the skull and another in regulating the skeletogenic fates of neural crest-derived mesenchyme. Developmental Dynamics 245: 947-962, 2016. (C) 2016 Wiley Periodicals, Inc.

Název v anglickém jazyce

BMP Signaling Regulates the Fate of Chondro-osteoprogenitor Cells in Facial Mesenchyme in a Stage-Specific Manner

Popis výsledku anglicky

Background: Lineage tracing has shown that most of the facial skeleton is derived from cranial neural crest cells. However, the local signals that influence postmigratory, neural crest-derived mesenchyme also play a major role in patterning the skeleton. Here, we study the role of BMP signaling in regulating the fate of chondro-osteoprogenitor cells in the face. Results: A single Noggin-soaked bead inserted into stage 15 chicken embryos induced an ectopic cartilage resembling the interorbital septum within the palate and other midline structures. In contrast, the same treatment in stage 20 embryos caused a loss of bones. The molecular basis for the stage-specific response to Noggin lay in the simultaneous up-regulation of SOX9 and downregulation of RUNX2 in the maxillary mesenchyme, increased cell adhesiveness as shown by N-cadherin induction around the beads and increased RA pathway gene expression. None of these changes were observed in stage 20 embryos. Conclusions: These experiments demonstrate how slight changes in expression of growth factors such as BMPs could lead to gain or loss of cartilage in the upper jaw during vertebrate evolution. In addition, BMPs have at least two roles: one in patterning the skull and another in regulating the skeletogenic fates of neural crest-derived mesenchyme. Developmental Dynamics 245: 947-962, 2016. (C) 2016 Wiley Periodicals, Inc.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental dynamics

ISSN

1058-8388

e-ISSN

—

Svazek periodika

245

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

947-962

Kód UT WoS článku

000384752100005

EID výsledku v databázi Scopus

2-s2.0-84981288403