Role of FZD6 in pathogenesis of canine acanthomatous epulis and human ameloblastoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F18%3A43876295" target="_blank" >RIV/62157124:16170/18:43876295 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Role of FZD6 in pathogenesis of canine acanthomatous epulis and human ameloblastoma

Popis výsledku v původním jazyce

Introduction: Acanthomatous epulis in dog and human ameloblastoma belongs to the group of odontogenic tumors. It was proposed that they arise from remnants of the dental lamina, where we observed high expression of Frizzled 6 (FZD6). FZD6 is a receptor of WNT signalling pathway, which is crucial for cell development, migration and polarity and its defects are linked to many cancers. Our aim is to evaluate expression of FZD6 in these odontogenic tumors. Materials and Methods: First, we used immunofluorescence to map the distribution of FZD6 positive cells in cancer tissue and its subcellular expression was analysed on the confocal microscope. Next, the alteration of gene expression of all main WNT pathway members was analysed in malign and benign form of tumour by PCR Array to uncover possible differences in upregulation of certain section of this pathway. Results: FZD6 was highly expressed in tumorous tissue. We observed a different distribution and expression pattern of FZD6 in dog acanthomatous epulis and human ameloblastoma. Moreover, we observed overexpression of several genes related to WNT in human ameloblastoma such as Axin2, Lef1, Tcf7 or Dkk3. Conclusions: We are suggesting, that WNT signalling pathway is involved in pathogenesis of canine acathomatous epulis as well as in human ameloblastoma. Nevertheless, different expression pattern and cell distribution is suggesting, that these tumors might have a different origin.

Název v anglickém jazyce

Role of FZD6 in pathogenesis of canine acanthomatous epulis and human ameloblastoma

Popis výsledku anglicky

Introduction: Acanthomatous epulis in dog and human ameloblastoma belongs to the group of odontogenic tumors. It was proposed that they arise from remnants of the dental lamina, where we observed high expression of Frizzled 6 (FZD6). FZD6 is a receptor of WNT signalling pathway, which is crucial for cell development, migration and polarity and its defects are linked to many cancers. Our aim is to evaluate expression of FZD6 in these odontogenic tumors. Materials and Methods: First, we used immunofluorescence to map the distribution of FZD6 positive cells in cancer tissue and its subcellular expression was analysed on the confocal microscope. Next, the alteration of gene expression of all main WNT pathway members was analysed in malign and benign form of tumour by PCR Array to uncover possible differences in upregulation of certain section of this pathway. Results: FZD6 was highly expressed in tumorous tissue. We observed a different distribution and expression pattern of FZD6 in dog acanthomatous epulis and human ameloblastoma. Moreover, we observed overexpression of several genes related to WNT in human ameloblastoma such as Axin2, Lef1, Tcf7 or Dkk3. Conclusions: We are suggesting, that WNT signalling pathway is involved in pathogenesis of canine acathomatous epulis as well as in human ameloblastoma. Nevertheless, different expression pattern and cell distribution is suggesting, that these tumors might have a different origin.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů