Wnt Signalling in Ameloblastoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F21%3A43879438" target="_blank" >RIV/62157124:16170/21:43879438 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Wnt Signalling in Ameloblastoma

Popis výsledku v původním jazyce

The wingless-related integration site pathway (WNT) is crucial for tissue homeostasis and its misregulation was described in numerous diseases including cancer. We focused on the expression and possible alteration of WNT signalling pathway in human ameloblastoma - the most common oral tumor with an odontogenic origin. Ameloblastoma is a benign tumor, but its invasive behaviour often makes the treatment complicated. We used immunohistochemistry and immunofluorescence to map the expression of key WNT members and PCR array to analyze gene expression of 84 WNT members in ameloblastoma samples. Our analysis revealed that members of canonical WNT signaling pathway such as EP300 and TCF7 were significantly upregulated. The most up regulated receptors in ameloblastoma were FZD1, FZD7 and co-receptor LRP5. On the other hand, our analysis also revealed significant upregulation of several genes associated with inhibition of WNT signaling such as SFRP1, or NLK in ameloblastoma. A number of WNT signaling target genes were also affected, with PITX2 and AXIN2 significantly upregulated and FOSL1 downregulated. Two planar cell polarity genes, VANGL2 and MAPK8 were upregulated, while DAAM1 was downregulated in ameloblastoma when compared to gingival tissues. In conclusion, our study provided deeper insight into gene expression profile of WNT pathway in ameloblastoma, opening up new avenues which should be tested for potential prediction of future cell behaviour in this neoplasm.

Název v anglickém jazyce

Wnt Signalling in Ameloblastoma

Popis výsledku anglicky

The wingless-related integration site pathway (WNT) is crucial for tissue homeostasis and its misregulation was described in numerous diseases including cancer. We focused on the expression and possible alteration of WNT signalling pathway in human ameloblastoma - the most common oral tumor with an odontogenic origin. Ameloblastoma is a benign tumor, but its invasive behaviour often makes the treatment complicated. We used immunohistochemistry and immunofluorescence to map the expression of key WNT members and PCR array to analyze gene expression of 84 WNT members in ameloblastoma samples. Our analysis revealed that members of canonical WNT signaling pathway such as EP300 and TCF7 were significantly upregulated. The most up regulated receptors in ameloblastoma were FZD1, FZD7 and co-receptor LRP5. On the other hand, our analysis also revealed significant upregulation of several genes associated with inhibition of WNT signaling such as SFRP1, or NLK in ameloblastoma. A number of WNT signaling target genes were also affected, with PITX2 and AXIN2 significantly upregulated and FOSL1 downregulated. Two planar cell polarity genes, VANGL2 and MAPK8 were upregulated, while DAAM1 was downregulated in ameloblastoma when compared to gingival tissues. In conclusion, our study provided deeper insight into gene expression profile of WNT pathway in ameloblastoma, opening up new avenues which should be tested for potential prediction of future cell behaviour in this neoplasm.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů