Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F19%3A43878408" target="_blank" >RIV/62157124:16170/19:43878408 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68407700:21720/19:00346786
Výsledek na webu
<a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_S399.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_S399.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934377" target="_blank" >10.33549/physiolres.934377</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats
Popis výsledku v původním jazyce
The laser radiation absorbed by cells induces production of reactive oxygen species (ROS), followed by the development of oxidative stress. Proteins are major targets for ROS due to their abundance in biological systems. The aim of the present pilot study was to examine the effects of transcutaneous laser blood irradiation (TLBI), i.e., low-level laser therapy (LLLT) at 830 nm on plasma proteome in Wistar rats. Rats were irradiated in the heart area (i.e. coronary arteries) daily (i.e., for 9-day period), by commercially available GaAsAl diode laser (Maestro/CCM, Medicom Prague, Czech Republic, lambda=830 nm, power density 450mW/cm(2), daily dose 60,3 J/cm(2), irradiation time 134 sec). The comparison of blood plasma proteome from irradiated and non-irradiated rats was performed utilizing 2D electrophoresis followed by MALDI TOF/TOF mass spectrometry. LLLT led to a quantitative change in the acute phase proteins with antioxidant protection i.e., haptoglobin (log(2) fold change (FC)=3.5), hemopexin (log(2) FC=0.5), fibrinogen gamma (log(2) FC= 1.4), alpha-1-antitrypsin (log(2) FC=-2.2), fetuin A (log(2) FC= -0.6) and fetuin B (log(2) FC=-2.3). In comparison to conventional biochemical methods, the changes in protein levels in blood plasma induced by LLLT offer a deeper insight into the oxidative stress response.
Název v anglickém jazyce
Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats
Popis výsledku anglicky
The laser radiation absorbed by cells induces production of reactive oxygen species (ROS), followed by the development of oxidative stress. Proteins are major targets for ROS due to their abundance in biological systems. The aim of the present pilot study was to examine the effects of transcutaneous laser blood irradiation (TLBI), i.e., low-level laser therapy (LLLT) at 830 nm on plasma proteome in Wistar rats. Rats were irradiated in the heart area (i.e. coronary arteries) daily (i.e., for 9-day period), by commercially available GaAsAl diode laser (Maestro/CCM, Medicom Prague, Czech Republic, lambda=830 nm, power density 450mW/cm(2), daily dose 60,3 J/cm(2), irradiation time 134 sec). The comparison of blood plasma proteome from irradiated and non-irradiated rats was performed utilizing 2D electrophoresis followed by MALDI TOF/TOF mass spectrometry. LLLT led to a quantitative change in the acute phase proteins with antioxidant protection i.e., haptoglobin (log(2) fold change (FC)=3.5), hemopexin (log(2) FC=0.5), fibrinogen gamma (log(2) FC= 1.4), alpha-1-antitrypsin (log(2) FC=-2.2), fetuin A (log(2) FC= -0.6) and fetuin B (log(2) FC=-2.3). In comparison to conventional biochemical methods, the changes in protein levels in blood plasma induced by LLLT offer a deeper insight into the oxidative stress response.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30105 - Physiology (including cytology)
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Physiological Research
ISSN
0862-8408
e-ISSN
1802-9973
Svazek periodika
68
Číslo periodika v rámci svazku
Suplement4
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
6
Strana od-do
"S399"-"S404"
Kód UT WoS článku
000529413800005
EID výsledku v databázi Scopus
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