Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21720%2F19%3A00346786" target="_blank" >RIV/68407700:21720/19:00346786 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/19:43878408

Výsledek na webu

<a href="https://doi.org/10.33549/PHYSIOLRES.934377" target="_blank" >https://doi.org/10.33549/PHYSIOLRES.934377</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/PHYSIOLRES.934377" target="_blank" >10.33549/PHYSIOLRES.934377</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats

Popis výsledku v původním jazyce

The laser radiation absorbed by cells induces production of reactive oxygen species (ROS), followed by the development of oxidative stress. Proteins are major targets for ROS due to their abundance in biological systems. The aim of the present pilot study was to examine the effects of transcutaneous laser blood irradiation (TLBI), i.e., low-level laser therapy (LLLT) at 830 nm on plasma proteome in Wistar rats. Rats were irradiated in the heart area (i.e. coronary arteries) daily (i.e., for 9-day period), by commercially available GaAsAl diode laser (Maestro/CCM, Medicom Prague, Czech Republic, λ=830 nm, power density 450mW/cm2, daily dose 60,3 J/ cm2, irradiation time 134 sec). The comparison of blood plasma proteome from irradiated and non-irradiated rats was performed utilizing 2D electrophoresis followed by MALDI TOF/TOF mass spectrometry. LLLT led to a quantitative change in the acute phase proteins with antioxidant protection i.e., haptoglobin (log2 fold change (FC)=3.5), hemopexin (log2 FC=0.5), fibrinogen gamma (log2 FC=1.4), alpha-1-antitrypsin (log2 FC=-2.2), fetuin A (log2 FC=-0.6) and fetuin B (log2 FC=-2.3). In comparison to conventional biochemical methods, the changes in protein levels in blood plasma induced by LLLT offer a deeper insight into the oxidative stress response.

Název v anglickém jazyce

Proteomic Analysis of Plasma Proteins after Low-Level Laser Therapy in Rats

Popis výsledku anglicky

The laser radiation absorbed by cells induces production of reactive oxygen species (ROS), followed by the development of oxidative stress. Proteins are major targets for ROS due to their abundance in biological systems. The aim of the present pilot study was to examine the effects of transcutaneous laser blood irradiation (TLBI), i.e., low-level laser therapy (LLLT) at 830 nm on plasma proteome in Wistar rats. Rats were irradiated in the heart area (i.e. coronary arteries) daily (i.e., for 9-day period), by commercially available GaAsAl diode laser (Maestro/CCM, Medicom Prague, Czech Republic, λ=830 nm, power density 450mW/cm2, daily dose 60,3 J/ cm2, irradiation time 134 sec). The comparison of blood plasma proteome from irradiated and non-irradiated rats was performed utilizing 2D electrophoresis followed by MALDI TOF/TOF mass spectrometry. LLLT led to a quantitative change in the acute phase proteins with antioxidant protection i.e., haptoglobin (log2 fold change (FC)=3.5), hemopexin (log2 FC=0.5), fibrinogen gamma (log2 FC=1.4), alpha-1-antitrypsin (log2 FC=-2.2), fetuin A (log2 FC=-0.6) and fetuin B (log2 FC=-2.3). In comparison to conventional biochemical methods, the changes in protein levels in blood plasma induced by LLLT offer a deeper insight into the oxidative stress response.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

<a href="/cs/project/LO1605" target="_blank" >LO1605: Univerzitní centrum energeticky efektivních budov – Fáze udržitelnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

1802-9973

Svazek periodika

68

Číslo periodika v rámci svazku

Suppl. 4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

"S399"-"S404"

Kód UT WoS článku

000529413800005

EID výsledku v databázi Scopus

2-s2.0-85081138479